Immunogenicity and clinical protection against equine influenza by DNA vaccination of ponies
Immunogenicity and clinical protection against equine influenza by DNA vaccination of ponies
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DOI：
10.1016/j.vaccine.2012.03.026
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发表时间：
2012-06-06
期刊：
影响因子：
5.5
通讯作者：
Rao, Srinivas S.
中科院分区：
文献类型：
--
作者：
Ault, Alida;Zajac, Alyse M.;Rao, Srinivas S.
马流感A(H3N8)病毒感染是引起马匹呼吸道疾病的主要原因，导致广泛的发病率和经济损失。与其他物种的流感一样，马流感毒株不断发生突变，往往需要开发新的疫苗。目前的灭活(灭活)疫苗虽然有效，但对不同的亚型只能提供有限的保护，需要经常加强免疫。研究新的疫苗技术，包括基于基因的疫苗，旨在增加保护性免疫的中和效力、广度和持续时间。在这里，我们证明了表达马H3N8流感病毒血凝素蛋白的DNA疫苗能产生同源和异种免疫反应，并对临床疾病和同源H3N8病毒在马身上的复制具有保护作用。此外，我们证明了无针分娩与使用针头和注射器的传统肠外注射一样有效和有效。这些发现表明，DNA疫苗为预防马流感的兽用疫苗提供了一种安全、有效和有前景的替代方法。爱思唯尔有限公司出版。
Equine influenza A (H3N8) virus infection is a leading cause of respiratory disease in horses, resulting in widespread morbidity and economic losses. As with influenza in other species, equine influenza strains continuously mutate, often requiring the development of new vaccines. Current inactivated (killed) vaccines, while efficacious, only offer limited protection against diverse subtypes and require frequent boosts. Research into new vaccine technologies, including gene-based vaccines, aims to increase the neutralization potency, breadth, and duration of protective immunity. Here, we demonstrate that a DNA vaccine expressing the hemagglutinin protein of equine H3N8 influenza virus generates homologous and heterologous immune responses, and protects against clinical disease and viral replication by homologous H3N8 virus in horses. Furthermore, we demonstrate that needle-free delivery is as efficient and effective as conventional parenteral injection using a needle and syringe. These findings suggest that DNA vaccines offer a safe, effective, and promising alternative approach for veterinary vaccines against equine influenza. Published by Elsevier Ltd.