REN1 is required for development of microconidia and macroconidia, but not of chlamydospores, in the plant pathogenic fungus Fusarium oxysporum
REN1 is required for development of microconidia and macroconidia, but not of chlamydospores, in the plant pathogenic fungus Fusarium oxysporum
复制标题
DOI：
10.1534/genetics.166.1.113
复制
发表时间：
2004-01-01
期刊：
影响因子：
3.3
通讯作者：
Tsuge, T
中科院分区：
文献类型：
--
作者：
Ohara, T;Inoue, I;Tsuge, T
丝状真菌尖孢镰刀菌是一种土传兼性寄生虫，在多种作物中造成重要的经济损失。F.尖孢菌在琼脂培养基上呈丝状生长，并进行无性发育，产生三种孢子：小分生孢子、大分生孢子和厚垣孢子。椭圆形的小分生孢子和镰刀形的大分生孢子由瓶梗通过向基分裂形成;厚壁的球形厚垣孢子由菌丝顶生或通过菌丝细胞的修饰形成。在这里，我们描述了rensa，一个分生孢子突变体的F。尖孢霉，通过限制性内切酶介导的整合诱变获得。对rensa的分子分析确定了受影响的基因REN 1，该基因编码的蛋白质与构巢曲霉的梅达和稻瘟病菌的Acr 1相似。推测梅达和Acr 1是这些真菌中参与分生孢子发生的转录调节因子。rensa突变体和REN 1靶菌株缺乏正常的分生孢子梗和瓶梗，并通过向顶分裂直接从菌丝形成杆状分生孢子样细胞。然而，这些突变体表现出正常的营养生长和厚垣孢子形成。使用表达Ren 1-绿色荧光蛋白融合体的菌株验证Ren 1的核定位。这些数据有力地表明，REN 1编码一个转录调节子，该转录调节子是真菌分生孢子发生细胞正确分化以发育小分生孢子和大分生孢子所必需的。尖孢菌属
The filamentous fungus Fusarium oxysporum is a soil-borne facultative parasite that causes economically important losses in a wide variety of crops. F. oxysporum exhibits filamentous growth on agar media and undergoes asexual development producing three kinds of spores: microconidia, macroconidia, and chlamydospores. Ellipsoidal microconidia and falcate macroconidia are formed from phialides by basipetal division; globose chlamydospores with thick walls are formed acrogenously from hyphae or by the modification of hyphal cells. Here we describe rensa, a conidiation mutant of F. oxysporum, obtained by restriction-enzyme-mediated integration mutagenesis. Molecular analysis of rensa identified the affected gene, REN1, which encodes a protein with similarity to MedA of Aspergillus nidulans and Acr1 of Magnaporthe grisea. MedA and Acr1 are presumed transcription regulators involved in conidiogenesis in these fungi. The rensa mutant and REN1-targeted strains lack normal conidiophores and phialides and form rod-shaped, conidium-like cells directly from hyphae by acropetal division. These mutants, however, exhibit normal vegetative growth and chlamydospore formation. Nuclear localization of Ren1 was verified using strains expressing the Ren1-green fluorescent protein fusions. These data strongly suggest that REN1 encodes a transcription regulator required for the correct differentiation of conidiogenesis cells for development of microconidia and macroconidia in F. oxysporum.