Expression of a gene encoding a unique protein-tyrosine kinase within specific fetal- and adult-derived hematopoietic lineages.
Expression of a gene encoding a unique protein-tyrosine kinase within specific fetal- and adult-derived hematopoietic lineages.
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编码独特的蛋白酪氨酸激酶的基因在特定的胎儿和成人造血谱系中的表达。
DOI：
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发表时间：
1993
影响因子：
11.1
通讯作者：
G. Keller
中科院分区：
文献类型：
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作者：
K. Choi;M. Kennedy;G. Keller
从未分化胚胎干细胞（ES）中分离到一个编码酪氨酸激酶蛋白的基因。Emsk（胚胎干细胞激酶）基因在许多不同的淋巴和髓系造血谱系中表达，并且已被证明与最近分离的局灶黏着相关激酶基因（Fadk）相同。在分析的非淋巴样谱系中，发现Emsk/Fadk在原始和终代红系细胞中表达，而在肥大细胞或巨噬细胞中不表达。所有CD5+ (B-1a) B细胞以及新分离的常规（B-2） B细胞均表达可检测水平的Emsk/Fadk。在t细胞谱系中，Emsk/Fadk在V γ 5 γ / δ细胞以及胸腺内未成熟的α / β细胞中表达。随着α / β t细胞群的成熟和退出胸腺，Emsk/Fadk的表达似乎被下调。这里概述的表达模式表明，Emsk/Fadk在造血发育的多个阶段发挥作用，并提出了由该基因编码的激酶具有比最初认为的更广泛的活性谱的可能性。
A gene encoding a unique protein-tyrosine kinase was isolated by PCR from undifferentiated embryonic stem (ES) cells. The gene, Emsk (embryonic stem cell kinase), is expressed in a number of different lymphoid and myeloid hematopoietic lineages and has been shown to be identical to the recently isolated focal adhesion-associated kinase gene (Fadk). Within the nonlymphoid lineages analyzed, Emsk/Fadk was found to be expressed in primitive and definitive erythroid cells but not in mast cells or macrophages. All CD5+ (B-1a) B cells tested, as well as freshly isolated conventional (B-2) B cells, expressed readily detectable levels of Emsk/Fadk. Within the T-cell lineage, Emsk/Fadk was expressed in V gamma 5 gamma/delta cells as well as in immature alpha/beta cells found within the thymus. As the alpha/beta T-cell population matures and exits the thymus, expression of Emsk/Fadk appears to be down regulated. The expression pattern outlined here suggests a role for Emsk/Fadk in multiple stages of hematopoietic development and raises the possibility that the kinase encoded by this gene has a broader spectrum of activities than was initially suggested.