Chromatin Preparation from Murine Eosinophils for Genome-Wide Analyses.
Chromatin Preparation from Murine Eosinophils for Genome-Wide Analyses.
复制标题
从鼠嗜酸性粒细胞中制备染色质用于全基因组分析。
DOI：
10.1007/978-1-4939-7896-0_20
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Fulkerson,PatriciaC
中科院分区：
文献类型：
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作者：
Bouffi,Carine;Barski,Artem;Fulkerson,PatriciaC
动态基因表达是指导造血谱系定型和分化的主要机制。最近的进展揭示了染色质特征与功能性遗传序列之间的关联，因此这些染色质特征可用于预测调控元件，例如可能指导谱系分化的增强子。我们对调节嗜酸性粒细胞发育的遗传元件的了解非常有限，这可能是由于处理罕见的复杂细胞的技术挑战所致。在此，我们描述了从小鼠骨髓中分选成熟嗜酸性粒细胞并制备可用于 ChIP 研究的染色质的方案，以对成熟嗜酸性粒细胞中的组蛋白标记和转录因子进行全基因组图谱。嗜酸性粒细胞发育关键阶段的全面表观基因组分析最终将有助于定义调节嗜酸性粒细胞产生所需的基因调控网络。
Dynamic gene expression is a major mechanism that directs hematopoietic lineage commitment and differentiation. Recent advances have revealed an association between chromatin signatures and functional genetic sequences such that these chromatin signatures can be used to predict regulatory elements such as enhancers that may direct lineage differentiation. Our understanding of the genetic elements that regulate eosinophil development is very limited, likely due to the technical challenges in working with a rare complex cell. Herein, we describe protocols to sort mature eosinophils from the bone marrow of mice and to prepare chromatin that can be used for ChIP studies for genome-wide mapping of histone marks and transcription factors in mature eosinophils. Comprehensive epigenomic profiling during critical stages in eosinophil development will ultimately aid in defining the gene regulatory networks necessary to regulate eosinophil production.