Respiratory coronavirus infections in children younger than two years of age
Respiratory coronavirus infections in children younger than two years of age
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DOI：
10.1097/00006454-200002000-00016
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发表时间：
2000-02-01
影响因子：
3.6
通讯作者：
Hovi, T
中科院分区：
文献类型：
--
作者：
Nokso-Koivisto, J;Pitkäranta, A;Hovi, T
传统的诊断方法。现在，RT-PCR已被引入用于流行病学研究和临床诊断目的。在本研究中，我们发现在2.4%的急性呼吸道感染和/或AOM患儿的NPA标本和3%的AOM患儿的MEF标本中可以检测到HCoV。2.4%的冠状病毒感染率低于之前对儿童的一些研究，2.3%可能是因为我们的孩子很小。事实上，几项血清学研究表明，2岁儿童感染HCoV的频率相对较低(抗体流行率为3%至8%)，但对于年龄较大的儿童和在可能每2至3年发生一次的流行病期间，发病率可上升至19%。5、11-13因此，观察到的感染的明显缺乏也可能反映了人口中的一种特定的流行病学情况。4、5在成人中也有不同的发病率。1，14根据血清学研究，几乎一半的HCoV感染是无症状的11，因此在本研究中可能仍未被识别。综上所述，我们采用了RT-PCR-杂交方法大规模检测呼吸道样本中的HCoV OC43和229E。对储存的NPA和MEF样本的初步分析显示，2.4%的样本感染了HCoV，其中约一半与AOM有关。这一结果证明了将这一检测添加到全面呼吸道病毒检测研究的诊断小组中是合理的。
tional diagnostic methods. Now RT-PCR has been introduced for epidemiologic studies and also for clinical diagnostic purposes. In the present study we found that HCoV were detectable by RT-PCR in 2.4% of NPA samples of children with acute respiratory infection and/or AOM and in 3% of MEF samples of children with AOM. The 2.4% coronavirus infection rate is lower than in some previous studies with children, 2, 3 possibly because our children were very young. Indeed several serologic studies suggest that the frequency of HCoV infections in children 2 years old is relatively low (antibody prevalence, 3 to 8%), but with older children and during the epidemics, which may occur every 2 to 3 years, the incidence can rise to 19%. 5, 11–13 Hence the apparent paucity of the observed infections may also reflect a specific epidemiologic situation in the population. 4, 5 Variable incidences have also been reported in adults. 1, 14 According to serologic studies almost one-half of the HCoV infections are asymptomatic11 and therefore could have remained unrecognized in the current study.In conclusion we have adopted an RT-PCR-hybridization assay for large scale testing of HCoV OC43 and 229E in respiratory specimens. A pilot analysis of stored NPA and MEF specimens revealed a HCoV infection in 2.4% of the specimens, about one-half of them collected in association with AOM. This result justifies the addition of this test to the diagnostic panel of comprehensive respiratory virus detection studies.