Prevalence of canine coronavirus antibodies by an enzyme-linked immunosorbent assay in dogs in the south of Italy
Prevalence of canine coronavirus antibodies by an enzyme-linked immunosorbent assay in dogs in the south of Italy
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DOI：
10.1016/s0166-0934(01)00450-5
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发表时间：
2002-04-01
影响因子：
3.1
通讯作者：
Buonavoglia, C
中科院分区：
文献类型：
--
作者：
Pratelli, A;Elia, G;Buonavoglia, C
以犬冠状病毒（CCoV）感染的CrFK细胞上清为抗原，建立了检测犬冠状病毒抗体的酶联免疫吸附试验（ELISA）。在109个犬只血清样本中，80个经常规病毒中和试验呈阳性的样本亦呈ELISA阳性。17份经病毒中和试验呈阴性的样本经Elisa和确证性Western印迹试验呈阳性。ELISA在检测CCoV抗体方面比病毒中和试验敏感得多，可用作病毒中和的替代技术。(C)2002 Elsevier Science B.V.保留所有权利。
An enzyme-linked immunosorbent assay (Elisa), using as antigen canine coronavirus-infected CrFK cell supernatant, was developed to detect antibodies against canine coronavirus (CCoV). Out of a total of 109 dog serum samples, 80 which were positive by routine virus neutralisation test were also Elisa positive. Seventeen samples which were negative by the virus neutralisation test, were positive by Elisa and by the confirmatory Western blotting test. The Elisa was substantially more sensitive than the virus neutralisation test in detecting antibodies to CCoV and may be used as an alternative technique to virus neutralisation. (C) 2002 Elsevier Science B.V. All rights reserved.