Multiplex mapping of CD4 T cell epitopes using class II tetramers
Multiplex mapping of CD4 T cell epitopes using class II tetramers
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DOI：
10.1016/j.clim.2006.03.008
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发表时间：
2006-07-01
影响因子：
8.6
通讯作者：
Kwok, William W.
中科院分区：
文献类型：
--
作者：
Yang, Junbao;James, Eddie A.;Kwok, William W.
随着II类四聚体技术的出现，四聚体引导的表位作图（TGEM）技术被开发用于鉴定CD4（+）T细胞表位。这允许直接鉴定由应答T细胞识别的表位，其限于单个感兴趣的MHC等位基因。然而，由于每个人都携带多个II类等位基因，因此设计一种方法来同时鉴定不同II类等位基因呈现的CD 4（+）表位将是有利的。在本研究中，开发了一种多重TGEM方法，以确定同时由多个HLA II类等位基因呈递的抗原表位。在这种新方法中，CD4+ T细胞用多组MHC II类四聚体染色，每组四聚体都用独特的荧光标记物标记。使用这种多重方法，从流感抗原血凝素和基质蛋白提出的多个II类等位基因的新表位，在一个单一的实验设置中进行了鉴定。(c)2006年爱思唯尔公司All rights reserved.
With the advent of class II tetramer technology, a tetramer-guided epitope mapping (TGEM) technique was developed for the identification of CD4(+) T cell epitopes. This allowed the direct identification of epitopes recognized by the responding T cells, which were restricted to the single MHC allele of interest. However, as each individual carries multiple class II alleles, it would be advantageous to design an approach to identify CD4(+) epitopes presented by different class II alleles at the same time. In the present study, a multiplex TGEM approach was developed to identify antigenic epitopes presented by multiple HLA class II alletes simultaneously. In this new approach, CD4+ T cells were stained with multiple sets of MHC class II tetramers-each labeled with a unique fluorescent Label. Using this multiplex approach, novel epitopes from influenza antigens hemagglutinin and matrix protein presented by multiple class II alleles were identified in a single experimental setting. (c) 2006 Elsevier Inc. All rights reserved.