Adaptive Multiphoton Endomicroscope Incorporating a Polarization-Maintaining Multicore Optical Fibre
Adaptive Multiphoton Endomicroscope Incorporating a Polarization-Maintaining Multicore Optical Fibre
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DOI：
10.1109/jstqe.2015.2488283
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发表时间：
2016-05-01
影响因子：
4.9
通讯作者：
French, Paul M. W.
中科院分区：
文献类型：
--
作者：
Kim, Youngchan;Warren, Sean C.;French, Paul M. W.
我们提出了一种没有远端光学器件或机械部件的激光扫描多光子内窥镜，它采用保偏 (PM) 多芯光纤来传输、聚焦和扫描超短脉冲辐射，以实现双光子激发荧光成像。我们从理论上证明，与具有相同几何形状的非保偏光纤相比，在我们的实验配置中使用保偏多芯光纤增强了焦点中实现的荧光激发强度，并通过基于数值波前传播的计算机模拟证实了这一点。在我们的实验系统中，空间光调制器（SLM）用于对输入到内窥镜光纤每个芯的光的相位进行编程，使得从光纤远端出现的辐射发生干涉以提供聚焦扫描激发光束。我们证明，当光纤受到 100 Hz 更新率的扰动时，SLM 可以对多芯光纤上的路径长度变化进行动态相位校正。
We present a laser scanning multiphoton endomicroscope with no distal optics or mechanical components that incorporates a polarization-maintaining (PM) multicore optical fibre to deliver, focus, and scan ultrashort pulsed radiation for two-photon excited fluorescence imaging. We show theoretically that the use of a PM multicore fibre in our experimental configuration enhances the fluorescence excitation intensity achieved in the focal spot compared to a non-PM optical fibre with the same geometry and confirm this by computer simulations based on numerical wavefront propagation. In our experimental system, a spatial light modulator (SLM) is utilised to program the phase of the light input to each of the cores of the endoscope fibre such that the radiation emerging from the distal end of the fibre interferes to provide the focused scanning excitation beam. We demonstrate that the SLM can enable dynamic phase correction of path-length variations across the multicore optical fibre whilst the fibre is perturbed with an update rate of 100 Hz.