REEMERGENCE OF THE FETAL PATTERN OF L-TYPE CALCIUM-CHANNEL GENE-EXPRESSION IN NONINFARCTED MYOCARDIUM DURING LEFT-VENTRICULAR REMODELING
REEMERGENCE OF THE FETAL PATTERN OF L-TYPE CALCIUM-CHANNEL GENE-EXPRESSION IN NONINFARCTED MYOCARDIUM DURING LEFT-VENTRICULAR REMODELING
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DOI：
10.1006/bbrc.1995.2705
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发表时间：
1995-11-22
影响因子：
3.1
通讯作者：
ELSHERIF, N
中科院分区：
文献类型：
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作者：
GIDHJAIN, M;HUANG, BY;ELSHERIF, N
心脏 L 型电压依赖性钙通道 (VDCC) 是心脏动作电位和兴奋收缩耦合的关键组成部分。本研究的目的是检测心肌梗死 (MI) 重塑大鼠左心室中 VDCC 通道 α-1 亚基的选择性剪接区域 Motif IV 的表达变化。 RNase 保护测定用于确定心肌梗塞后 21 天非梗塞肥厚心室心肌中异构体表达的变化。我们的研究表明，心脏肥大与胎儿同种型 mRNA 水平的显着增加有关，同时胎儿：成人同种型比率会逆转为胎儿表型。 MI 重塑后心室中异构体表达的变化（之前未曾报道）是心脏肥大的相关遗传标志。 (C) 1995 学术出版社
The cardiac L-type voltage-dependent calcium channel (VDCC) is a critical component of cardiac action potential and excitation-contraction coupling. The objective of the present study was to examine the changes in expression in Motif IV, an alternatively spliced region of the alpha-1 subunit of the VDCC channel in post-myocardial infarction (MI) remodeled rat left ventricle. RNase protection assay was used to determine alteration in isoform expression in the noninfarcted hypertrophied ventricular myocardium 21 days post myocardial infarction. Our study demonstrates that cardiac hypertrophy is associated with significant increase in the mRNA level of the fetal isoform, with the reversion of fetal:adult isoform ratio to the fetal phenotype. Changes in isoform expression in the post-MI remodeled ventricle, not previously reported, is a pertinent genetic marker of cardiac hypertrophy. (C) 1995 Academic Press, Inc.