The joys of in vitro selection: chemically dressing oligonucleotides to satiate protein targets
The joys of in vitro selection: chemically dressing oligonucleotides to satiate protein targets
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DOI：
10.1016/s1367-5931(97)80103-2
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发表时间：
1997-06-01
影响因子：
7.8
通讯作者：
Eaton, BE
中科院分区：
文献类型：
--
作者：
Eaton, BE
寡核苷酸体外选择为蛋白质靶标提供了令人惊讶的特异性适体。已经开发了用于修饰核苷酸的合成化学来增强适体结合亲和力。适合三磷酸合成或亚磷酰胺活化的核苷的多样性现在足以与任何寡聚组合文库相媲美。
Oligonucleotide in vitro selection provides surprisingly specific aptamers to protein targets. Synthetic chemistries for modifying nucleotides have been developed to enhance aptamer binding affinity. The diversity of nucleosides amenable to either triphosphate synthesis or phosphoramidite activation is now sufficiently broad to rival any oligomeric combinatorial library.