Physiological and Proteomic Evidence for the Interactive Effects of Post‐Anthesis Heat Stress and Elevated CO2 on Wheat
Physiological and Proteomic Evidence for the Interactive Effects of Post‐Anthesis Heat Stress and Elevated CO2 on Wheat
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DOI：
10.1002/pmic.201800262
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发表时间：
2018-11
期刊：
影响因子：
3.4
通讯作者：
Xiaxiang Zhang;P. Högy;X. Wu;I. Schmid;Xiulin Wang;W. Schulze;D. Jiang;A. Fangmeier
中科院分区：
文献类型：
--
作者：
Xiaxiang Zhang;P. Högy;X. Wu;I. Schmid;Xiulin Wang;W. Schulze;D. Jiang;A. Fangmeier
CO2浓度升高促进叶片光合作用，提高作物产量。然而，作为一种主要的人为温室气体，CO2导致更频繁和严重的热胁迫，威胁作物生产力。CO2浓度升高和热应激的综合影响是复杂的，其潜在机制尚不清楚。在本研究中，研究了CO2浓度升高和高温对两种CO2浓度（380和550 µmol mol−1）和两种温度条件（环境和开花后热胁迫）下生长的春小麦叶片生理性状和蛋白质组的影响。CO2浓度升高增加叶片光合特性，生物量和粮食产量，而热胁迫抑制光合作用和产量。两种CO2浓度下温度对叶绿素含量和产量的影响差异不显著。叶片蛋白质组的分析表明，参与光合作用以及抗氧化剂和蛋白质合成途径的蛋白质显着下调，由于CO2浓度升高和热胁迫的组合。相应地，用升高的CO2和热胁迫处理的植物表现出降低的绿色叶面积、光合速率、抗氧化酶活性和1000粒重。目前的研究表明，未来的花后高温事件将减少二氧化碳升高的积极影响，并对小麦产量产生负面影响。
Elevated CO2 promotes leaf photosynthesis and improves crop grain yield. However, as a major anthropogenic greenhouse gas, CO2 contributes to more frequent and severe heat stress, which threatens crop productivity. The combined effects of elevated CO2 and heat stress are complex, and the underlying mechanisms are poorly understood. In the present study, the effects of elevated CO2 and high‐temperature on foliar physiological traits and the proteome of spring wheat grown under two CO2 concentrations (380 and 550 µmol mol−1) and two temperature conditions (ambient and post‐anthesis heat stress) are examined. Elevated CO2 increases leaf photosynthetic traits, biomass, and grain yield, while heat stress depresses photosynthesis and yield. Temperature‐induced impacts on chlorophyll content and grain yield are not significantly different under the two CO2 concentrations. Analysis of the leaf proteome reveals that proteins involved in photosynthesis as well as antioxidant and protein synthesis pathways are significantly downregulated due to the combination of elevated CO2 and heat stress. Correspondingly, plants treated with elevated CO2 and heat stress exhibit decreased green leaf area, photosynthetic rate, antioxidant enzyme activities, and 1000‐kernel weight. The present study demonstrates that future post‐anthesis heat episodes will diminish the positive effects of elevated CO2 and negatively impact wheat production.