Approaches to comprehensive multidimensional liquid chromatography systems
Approaches to comprehensive multidimensional liquid chromatography systems
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DOI：
10.1016/j.chroma.2008.12.073
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发表时间：
2009-02-27
影响因子：
4.1
通讯作者：
Guiochon, Georges
中科院分区：
文献类型：
--
作者：
Fairchild, Jacob N.;Horvath, Krisztian;Guiochon, Georges
这项工作比较了实现二维液相色谱 (2D-LC) 的三种不同方案的性能，包括它们可以生成的峰容量以及完成二维分析所需的时间。我们详细讨论这些二维液相色谱 x 液相色谱 (LC x LC) 方案如何花费时间以及如何比较它们。在保持第一维分离的特征不变的情况下，我们系统地改变了第二维分离的特征及其与第一维的耦合。在此过程中，根据综合二维液相色谱的三种已知实施方式的原理，创建了五个系统。这项工作展示了一种根据所需峰容量和时间限制选择最佳综合二维液相色谱方法的原始方法。使用 2D-LC 方法的决定源于需要在尽可能短的时间内达到给定的分辨率（即目标峰容量）或在给定的分析时间内达到尽可能高的分辨率。使用最合适的方案，我们建议如何在 20 分钟内产生 266 的峰值容量，或在 2.3 小时内产生约 2800 的峰值容量。当二维分离可用的时间非常短并且一维液相色谱无法达到所需的峰容量时，在线二维液相色谱方法无疑是最好的。然而，如果可以接受更长的分析时间，则只需将总分析时间增加 7 倍，即可将峰容量增加 10 倍。 (C) 2008 Elsevier B.V. 保留所有权利。
This work compares the performance of the three different schemes implementing two-dimensional liquid chromatography (2D-LC) in terms of the peak capacity that they can generate and of the time that they need to complete a two-dimensional analysis. We discuss in detail how time is spent in these two-dimensional liquid chromatography x liquid chromatography (LC x LC) schemes and how to compare them. Keeping constant the characteristics of the first-dimension separation, we systematically varied those of the second-dimension separation and of its coupling to the first-dimension. In the process, five systems were created, based on the principles of the three known implementations of comprehensive 2D-LC. This work demonstrates an original method for the selection of the best comprehensive 2D-LC approach, depending on the desired peak capacity and on time constraints. The decision to use a 2D-LC method arises from the need to achieve a given resolution (i.e., a target peak capacity) within as short a time as possible or to reach the highest possible resolution in a given analysis time. Using the most appropriate schemes, we suggest how it is realistically possible to generate peak capacities ranging from 266 in just over 20 min or about 2800 in 2.3 h. When the time available for a two-dimensional separation is very short and the desired peak capacity cannot be achieved in 1D-LC, an on-line 2D-LC approach is unquestionably best. However, if a longer analysis time is acceptable, a 10-fold increase in the peak capacity can be obtained at the cost of a mere 7-fold increase in total analysis time. (C) 2008 Elsevier B.V. All rights reserved.