Monoclonal antibodies to bovine blood group antigens.
Monoclonal antibodies to bovine blood group antigens.
复制标题
针对牛血型抗原的单克隆抗体。
DOI：
10.1111/j.1365-2052.1986.tb03183.x
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发表时间：
1986
期刊：
影响因子：
2.4
通讯作者：
L. Kilgour
中科院分区：
文献类型：
--
作者：
E. M. Tucker;L. Méténier;J. Grosclaude;S. Clarke;L. Kilgour
将小鼠骨髓瘤细胞与用牛红细胞免疫的小鼠的脾细胞融合制备杂交瘤。建立了16株能分泌与A、B、F、Z和S血型系统抗原反应的溶血性单克隆抗体的克隆株，其中一株抗体鉴定了B系统中的一种新因子。对1000只动物的红细胞进行的广泛试验表明，其中几种抗体适用于常规血型分型;其他抗体可能用于牛血型系统的遗传研究。
Hybridomas were made by fusing mouse myeloma cells with spleen cells from mice immunized with bovine red cells. Sixteen cloned lines which secreted haemolytic monoclonal antibodies reacting with antigens in the A, B, F, Z and S blood group systems were established; one of the antibodies identified a new factor in the B system. Extensive tests on red cells from 1000 animals indicated that several of the antibodies are suitable for use in routine blood typing; others are of potential use for genetic studies of the bovine blood group systems.