Apoptosis and proliferation in the neonatal murine heart
Apoptosis and proliferation in the neonatal murine heart
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DOI：
10.1002/dvdy.1139
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发表时间：
2001-07-01
影响因子：
2.5
通讯作者：
Shohet, RV
中科院分区：
文献类型：
--
作者：
Fernandez, E;Siddiquee, Z;Shohet, RV
在定时胎儿，新生儿和成年小鼠心脏的凋亡和增殖的空间和时间模式已确定使用原位末端标记技术检测片段化的DNA，和溴脱氧尿苷免疫荧光作为标记的DNA合成。此外，心脏表达的糖尿病相关蛋白进行了评估，免疫荧光。在发育中的传导系统区域的右心室心内膜下和基底隔中发现了明显的细胞凋亡标记。在右心室中，凋亡标记在产后第一天晚些时候激增，然后在产后8.5天下降到与左心室相似的水平。在基底隔的凋亡标记是最大的围产期，并逐渐下降到产后8.5天在心脏的其余部分看到的水平。停止增殖并没有同时发生在整个新生儿心脏。通过产后第4.5天，掺入BrdU是更大的左心室比右心室，特别是在subendocardium。Bax和Fas，促凋亡蛋白，在新生儿的两个心室均匀检测，而Bcl-2，抗凋亡蛋白，是检测不到的。这些数据表明，出生后心脏重塑的结果从细胞凋亡和增殖的变化。此外，这些过程的时间和空间模式，与分娩相关的血流动力学变化相一致，表明这两个过程可能受到机械因素的调节。(C)2001 Wiley-Liss，Inc.
The spatial and temporal patterns of apoptosis and proliferation in timed fetal, neonatal, and adult murine hearts have been determined using an in situ end-labeling technique for detecting fragmented DNA, and bromodeoxyuridine immunofluorescence as a marker for DNA synthesis. Also, cardiac expression of apoptosis-related proteins was assessed by immunofluorescence. Prominent apoptotic labeling was found in the right ventricular subendocardium and the basal septum in the area of the developing conduction system. In the right ventricle, apoptotic labeling surged late in the first day postpartum, then declined to levels similar to the left ventricle by postpartum day 8.5. Apoptotic labeling at the basal septum was greatest peripartum and gradually declined to levels seen in the rest of the heart by postpartum day 8.5. Cessation of proliferation did not occur simultaneously throughout the neonatal heart. Through postpartum day 4.5, incorporation of BrdU was greater in the left ventricle than in the right ventricle, particularly in the subendocardium. Bax and Fas, proapoptotic proteins, were detected homogeneously throughout both ventricles in the neonate, while Bcl-2, an antiapoptotic protein, was not detectable. These data suggest that postnatal cardiac remodeling results from changes in both apoptosis and proliferation. Furthermore, the temporal and spatial pattern of these processes, coincident with the hemodynamic changes associated with parturition, suggests that both processes may be regulated by mechanical factors. (C) 2001 Wiley-Liss, Inc.