Positioning, displacement, and localization of cells using ultrasonic forces
Positioning, displacement, and localization of cells using ultrasonic forces
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DOI：
10.1002/bit.20540
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发表时间：
2005-10-05
影响因子：
3.8
通讯作者：
Dual, J
中科院分区：
文献类型：
--
作者：
Haake, A;Neild, A;Dual, J
本文提出了一种定位和移动细胞的方法和装置。细胞悬浮在器件和任意表面（例如载玻片或晶片）之间的流体层中。该装置以超声波频率振动，在流体层中产生压力场。该压力场产生能够定位细胞的力场。根据设备的激发方式，可以生成 2D 或 3D 力场，将细胞分别定位在直线或点上。此外，随后可以以微米精度移动细胞。这已通过 HL60 和 MCF10A 细胞得到证实，并且可以在不损坏细胞的情况下实现。 (c) 2005 年 Wiley 期刊公司。
This paper presents a method and a device to position and displace cells. The cells are suspended in a fluid layer trapped between the device and an arbitrary surface such as an object slide or a wafer. The device vibrates at ultrasonic frequencies causing a pressure field in the fluid layer. This pressure field results in a force-field capable of positioning cells. Depending on the way in which the device is excited a 2-D or 3-D force-field can be generated, positioning the cells in lines or points respectively. Furthermore, it is possible to subsequently displace the cells with micrometer accuracy. This has been demonstrated using HL60 and MCF10A cells, and can be achieved without causing damage to the cells. (c) 2005 Wiley Periodicals, Inc.