Astrocyte stellation induced by Rho kinase inhibitors in culture
Astrocyte stellation induced by Rho kinase inhibitors in culture
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DOI：
10.1016/s0165-3806(03)00096-8
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发表时间：
2003-06-12
期刊：
影响因子：
--
通讯作者：
Misawa, M
中科院分区：
文献类型：
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作者：
Abe, K;Misawa, M
为了了解Rho激酶在星形胶质细胞形态调控中的作用，我们研究了Rho激酶抑制剂对培养的大鼠皮质星形胶质细胞形态的影响。培养的星形胶质细胞在没有刺激的情况下呈现扁平的多边形形态。但经选择性Rho激酶抑制剂Y-27632 （1-10 muM）处理后转变为带有过程的星状细胞。秋水仙碱可消除y -27632诱导的星形胶质细胞星形，表明该反应需要细胞骨架元件的重组。Y-27632的作用可被另一种Rho激酶抑制剂HA1077模仿，但不能被蛋白激酶C抑制剂GF-109203X或蛋白激酶A抑制剂KT5720模仿。这些结果表明，Rho激酶在没有刺激的情况下处于激活状态，并有助于维持培养的星形胶质细胞的多边形形态。(C) 2003 Elsevier Science B.V.版权所有
To understand the role of Rho kinases in regulation of astrocyte morphology, we investigated the effects of Rho kinase inhibitors on the morphology of cultured rat cortical astrocytes. Cultured astrocytes exhibited flattened, polygonal morphology in the absence of stimulation. but changed into process-bearing stellate cells following treatment with the selective Rho kinase inhibitor Y-27632 (1-10 muM). The Y-27632-induced astrocyte stellation was abolished by treatment with colchicine, indicating that the response requires reorganization of cytoskeletal elements. The effect of Y-27632 was mimicked by another Rho kinase inhibitor HA1077, but not by the protein kinase C inhibitor GF-109203X or the protein kinase A inhibitor KT5720. These results suggest that Rho kinases are in an activated state in the absence of stimuli and contribute to the maintenance of polygonal morphology of cultured astrocytes. (C) 2003 Elsevier Science B.V. All rights reserved.