Irradiation induces release of von Willebrand protein from endothelial cells in culture.
Irradiation induces release of von Willebrand protein from endothelial cells in culture.
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DOI：
10.1016/0360-3016(84)90742-9
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发表时间：
1984-10
期刊：
影响因子：
20.3
通讯作者：
L. Sporn;P. Rubin;V. Marder;D. Wagner
中科院分区：
文献类型：
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作者：
L. Sporn;P. Rubin;V. Marder;D. Wagner
用0 - 40戈伊剂量照射组织培养中的人脐静脉内皮细胞，并对释放的von Willebrand（vW）蛋白和保持与细胞结合的蛋白进行定量。20戈伊及更高剂量产生分泌的vW蛋白量的统计学显著增加。无论细胞在整个实验过程中连续标记还是在照射前预先标记，这种释放都存在。未观察到纤连蛋白分泌增加。对辐射的释放反应缓慢，在辐射后接近24小时达到显著性。vW蛋白的释放不是由于细胞裂解，因为分泌的vW蛋白含有非常少的细胞裂解物中存在的大的260千道尔顿vW前体亚基，并且通过免疫荧光染色细胞看起来是完整的。
Human umbilical vein endothelial cells in tissue culture were irradiated with doses between 0 and 40 Gy, and the released von Willebrand (vW) protein and that which remained associated with the cells was quantitated. Doses of 20 Gy and higher produced a statistically significant increase in amount of vW protein secreted. This release was present whether the cells were labeled continuously throughout the experiment or just prelabeled before irradiation. An increase in fibronectin secretion was not observed. The release response to radiation was slow, reaching significance close to 24 hours after irradiation. The release of vW protein was not due to cell lysis, because the secreted vW protein contained very little of the large 260- kilodalton vW precursor subunit present in cell lysates and the cells appeared intact by immunofluorescence staining.