Mapping QTLs on chicken chromosome 1 for performance and carcass traits in a broiler x layer cross
Mapping QTLs on chicken chromosome 1 for performance and carcass traits in a broiler x layer cross
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DOI：
10.1111/j.1365-2052.2005.01387.x
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发表时间：
2006-04-01
期刊：
影响因子：
2.4
通讯作者：
Coutinho, LL
中科院分区：
文献类型：
--
作者：
Nones, K;Ledur, MC;Coutinho, LL
为了定位影响生产性能和屠宰性状的数量性状基因座(QTL)，利用肉鸡品系和蛋鸡品系杂交建立了F-2鸡群体。将21个全同胞家系2063只F-2代雏鸡饲养为肉鸡，在42日龄时屠宰。测定了17个生产性能和屠体性状。利用鸡1号染色体上的80个微卫星对亲本(F-0)和F-1个体进行了基因分型，以筛选出信息丰富的标记。用33个信息标记对42日龄体重极端表型的F-2个体(BW42)进行选择性基因分型。利用26个标记对7个全同胞家系(649只F-2雏鸡)进行了基因分型。影响体重、采食量、屠体重量、鼓部和大腿重量以及腹部脂肪重量的数量性状基因座被定位到已经在其他群体中发现的区域。在1号染色体上定位了以前未见报道的与肌胃、肝、肺、心、脚的重量以及肠长相关的数量性状基因座。该F-2群体可用于发现新的QTL，为家禽生长和屠体性状相关基因的研究提供了新的资源。
With the objective of mapping quantitative trait loci (QTLs) for performance and carcass traits, an F-2 chicken population was developed by crossing broiler and layer lines. A total of 2063 F-2 chicks in 21 full-sib families were reared as broilers and slaughtered at 42 days of age. Seventeen performance and carcass traits were measured. Parental (F-0) and F-1 individuals were genotyped with 80 microsatellites from chicken chromosome 1 to select informative markers. Thirty-three informative markers were used for selective genotyping of F-2 individuals with extreme phenotypes for body weight at 42 days of age (BW42). Based on the regions identified by selective genotyping, seven full-sib families (649 F-2 chicks) were genotyped with 26 markers. Quantitative trait loci affecting body weight, feed intake, carcass weight, drums and thighs weight and abdominal fat weight were mapped to regions already identified in other populations. Quantitative trait loci for weights of gizzard, liver, lungs, heart and feet, as well as length of intestine, not previously described in the literature were mapped on chromosome 1. This F-2 population can be used to identify novel QTLs and constitutes a new resource for studies of genes related to growth and carcass traits in poultry.