CRISPR/Cas9-Mediated Editing of Genes Encoding rgs-CaM-like Proteins in Transgenic Potato Plants.
CRISPR/Cas9-Mediated Editing of Genes Encoding rgs-CaM-like Proteins in Transgenic Potato Plants.
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CRISPR/Cas9 介导的转基因马铃薯植物中编码 rgs-CaM 样蛋白的基因编辑。
DOI：
10.1007/978-1-4939-9635-3_9
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发表时间：
2019
影响因子：
--
通讯作者：
K. Nakahara
中科院分区：
文献类型：
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作者：
Z. Osmani;S. Jin;Masafumi Mikami;M. Endo;H. Atarashi;K. Fujino;Tetsuya Yamada;K. Nakahara
一种烟草钙调素样蛋白rgs-CaM已被证明以多种方式与病毒相互作用；它有助于双病毒感染，但也参与对黄瓜花叶病毒的启动免疫。序列相似性搜索在拟南芥和其他茄科植物（包括马铃薯）中发现了几种类似于rgs-CaM （rCML）的钙调素样蛋白。为了分析每个rCML的功能，使用CRISPR/Cas9系统将突变引入马铃薯rCML。在这里，我们描述了我们在稳定转化的马铃薯植物中CRISPR/ cas9介导的靶向诱变方案。
A tobacco calmodulin-like protein, rgs-CaM, has been shown to interact with viruses in a variety of ways; it contributes to geminivirus infections but is also involved in primed immunity to the cucumber mosaic virus. Sequence similarity searches revealed several calmodulin-like proteins similar to rgs-CaM (rCML) in Arabidopsis and other Solanaceae plants, including potato (Solanum tuberosum). To analyze the functions of each rCML, mutations were introduced into potato rCMLs using the CRISPR/Cas9 system. Here, we describe our protocol of the CRISPR/Cas9-mediated targeted mutagenesis in stably transformed potato plants.