Effect of diclazuril on intestinal morphology and SIgA expression in chicken infected with Eimeria tenella
Effect of diclazuril on intestinal morphology and SIgA expression in chicken infected with Eimeria tenella
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地克珠利对柔嫩艾美耳球虫感染鸡肠道形态及SIgA表达的影响
DOI：
10.1007/s00436-014-4074-7
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发表时间：
2014-11-01
影响因子：
2
通讯作者：
Wang, Hong-wei
中科院分区：
文献类型：
--
作者：
Tian, Er-jie;Zhou, Bian-hua;Wang, Hong-wei
分泌型免疫球蛋白A（SIgA）作为一种重要的黏膜免疫因子，在机体抵抗细菌、病毒、寄生虫等多种病原性感染中发挥重要作用。柔嫩艾美耳球虫（Eimeria tenella）是一种专性细胞内顶复体寄生虫，与消化道粘膜接触，特别寄生于鸡盲肠，引起严重的球虫病。球虫病目前主要使用化学治疗剂来控制。地克珠利是一种经典的抗球虫药，在家禽业中应用广泛。由于耐药问题的日益严重，因此了解地克珠利抗大肠杆菌作用中SIgA表达的模式至关重要。tenella。本研究采用HE染色和免疫组化技术观察了盲肠内SIgA的表达。同时，还对十二指肠、空肠和回肠组织进行了评价。HE染色结果显示，E.鸡柔嫩艾美耳球虫感染引起严重的损伤，表现为盲肠结构紊乱、出血、炎性细胞浸润、浆液性和粘液性渗出，十二指肠、空肠和回肠出现肉眼不可见的损伤。地克珠利治疗后，盲肠损伤明显减轻。免疫组化结果显示，感染组十二指肠、空肠和回肠SIgA含量均较对照组显著升高（P < 0.05），盲肠SIgA含量显著降低（P < 0.01）。有趣的是，与感染组相比，感染/地克珠利组的十二指肠（p < 0.05）、空肠和回肠中的SIgA水平降低，但盲肠中的SIgA水平升高（p < 0.05）。结果表明，地克珠利能有效减轻E. tenella，并通过提高鸡的免疫功能来治疗球虫病。
Secretory immunoglobulin A (SIgA), as a vital actor involving in the mucosal immunity, plays a key role in defending a variety of pathogenic infections, such as bacteria, viruses and parasites. Eimeria tenella is an obligate intracellular apicomplexan parasite contacting with the digestive tract mucosa and specially parasitizes chicken caecum, causing a severe form of coccidiosis. Coccidiosis is currently mainly controlled using chemotherapeutic agents. Diclazuril, a classic coccidiostat, was used widely in the poultry industry. Because of the rising problem of drug resistance, it is therefore crucial to understand the pattern of the SIgA expression in the action of diclazuril against E. tenella. In this study, the intestinal morphology in the caecum was analyzed by haematoxylin-eosin (HE) staining, and the SIgA expression was examined by immunohistochemical technique. At the same time, the duodenum, jejunum and ileum tissues have also been evaluated. HE staining results showed that E. tenella infection caused severe damage characterized by structural disorder, haemorrhage, inflammatory cell infiltration, serous and fibrinous exudation in chicken caecum and invisible damage in the duodenum, jejunum and ileum. With the treatment of diclazuril, the damage in the caecum was alleviated obviously. Immunohistochemical analysis demonstrated that the SIgA level in the infected group was increased in the duodenum (p < 0.05), jejunum and ileum, respectively, but decreased (p < 0.01) in the caecum, compared with the control group. Interestingly, the SIgA level was decreased in the duodenum (p < 0.05), jejunum and ileum but increased (p < 0.05) in the caecum in the infected/diclazuril group in comparison to the infected group. The results showed that diclazuril effectively alleviated the damage in the caecum induced by E. tenella and provided a cure for coccidiosis by improving the immune function in chickens.