Specific T cell recognition of peptides derived from prostate-specific antigen in patients with prostate cancer
Specific T cell recognition of peptides derived from prostate-specific antigen in patients with prostate cancer
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DOI：
10.1016/s0090-4295(97)00480-9
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发表时间：
1998-01-01
期刊：
影响因子：
2.1
通讯作者：
Celis, E
中科院分区：
文献类型：
--
作者：
Alexander, RB;Brady, F;Celis, E
目的：确定前列腺癌患者的T细胞是否能识别良性和恶性前列腺上皮中表达的蛋白。我们检测了7例HLA-AZ前列腺癌患者的T细胞对前列腺特异性抗原（PSA）的反应性。化学合成了从PSA中提取的四种pep tide，并显示它们与HLA-A2结合。作为对照，我们还检测了与HLA-A2结合的免疫原性流感基质肽Flu（58-66）。这些多肽通过体外刺激的方法刺激外周血淋巴细胞。在1例患者中，观察到特异性识别肽PSA（141-150）。其余6例患者对任何psa衍生肽均无反应性。特异性识别多肽PSA的T细胞系（141-150）在感染重组PSA痘苗病毒构建物后，不能识别表达内源性PSA的自体B细胞母细胞系。7例患者中有3例表现出对流感的特异性反应（58-66）。结论：我们在7例前列腺癌患者中发现1例特异性识别一种psa衍生肽。肽特异性淋巴细胞系不识别内源性PSA，提示该肽可能不是由产生PSA的前列腺癌细胞产生的，特异性识别PSA肽在我们的前列腺癌患者中并不常见。这种活性是否可以通过疫苗接种策略诱导，或者是否可以治疗已确诊前列腺癌的男性，仍有待证实。(C) 1998, Elsevier Science Inc.；版权所有。
Objectives, To determine if proteins known to be expressed by both benign and malignant prostate epithelium can be recognized by T cells from patients with prostate cancer. We examined 7 HLA-AZ patients with prostate cancer for evidence of T cell reactivity with prostate-specific antigen (PSA).Methods. Four pep tides derived from PSA were chemically synthesized and shown to bind to HLA-A2. As a control, we also examined the immunogenic influenza matrix peptide Flu(58-66) that binds to HLA-A2. These peptides were used to stimulate peripheral blood lymphocytes by in vitro stimulation.Results. In 1 patient, specific recognition of peptide PSA(141-150) was observed. The remaining 6 patients had no reactivity with any PSA-derived peptide. The T cell line with specific recognition of peptide PSA(141-150) failed to recognize an autologous B cell blast: line expressing endogenous PSA following infection with a recombinant PSA vaccinia virus construct. Three of the 7 patients demonstrated specific reactivity with Flu(58-66).Conclusions, We found specific recognition of one PSA-derived peptide in 1 patient-of 7 with prostate cancer. The peptide-specific lymphocyte cell line did not recognize endogenous PSA, suggesting that the peptide may not be produced by prostate cancer cells producing PSA, Specific recognition of PSA peptides was not common in our patients with prostate cancer. Whether such activity can be induced by vaccination strategies or can be therapeutic in men with established prostate cancer remains to be demonstrated. (C) 1998, Elsevier Science Inc. All rights reserved.