Expression of aromatase, estrogen receptor α and β, androgen receptor, and cytochrome P-450scc in the human early prepubertal testis
Expression of aromatase, estrogen receptor α and β, androgen receptor, and cytochrome P-450scc in the human early prepubertal testis
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DOI：
10.1203/01.pdr.0000246072.04663.bb
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发表时间：
2006-12-01
影响因子：
3.6
通讯作者：
Belgorosky, Alicia
中科院分区：
文献类型：
--
作者：
Berensztein, Esperanza B.;Baquedano, Maria Sonia;Belgorosky, Alicia
研究了芳香化酶、雌激素受体α（ER α）和β（ER β）、雄激素受体（AR）和细胞色素P-450侧链裂解酶（cP 450（SCC））在青春期前睾丸中的表达。样本分为三个年龄组（GR）：GR 1，新生儿（1至21天的新生儿，n = 5）; GR 2，出生后激活阶段（1至7个月大的婴儿，n = 6）; GR 3，儿童（12至60个月大的男孩，n = 4）。在所有细胞中通过免疫组织化学和mRNA表达观察到ER α的缺失或非常差的检测。GR 1和GR 2的间质细胞（LC）显示芳香化酶和cP 450（SCC）的强免疫染色，而ER β和AR的弱染色。间质细胞（IC）和支持细胞（SC）表达ER β，特别是在GR 1和GR 2中。肾小管周细胞（PC）AR表达强。对于所有标记物，GR 3中的表达最弱。在生殖细胞（GC），即生殖母细胞和精原细胞，芳香化酶和ER β的免疫表达强烈，而没有ER α，AR，或cP 450 SCC的表达被检测到。在新生儿和婴儿睾丸中，睾酮作用于PC可能调节婴儿LC的分化，而SC中AR的缺乏阻止了精子发生的发育。雌激素的作用不太清楚，但它可以调节前体LC和GC的足够池的保存。
The expression of aromatase, estrogen receptor a (ER alpha) and beta (ER beta), androgen receptor (AR), and cytochrome P-450 side chain cleavage enzyme (cP450(scc)) was studied in prepubertal testis. Samples were divided in three age groups (GRs): GR1, newborns (1- to 21-d-old neonates, n = 5); GR2, postnatal activation stage (1- to 7-mo-old infants, n = 6); GR3, childhood (12- to 60-mo-old boys, n = 4). Absent or very poor detection of ER alpha by immunohistochemistry in all cells and by mRNA expression was observed. Leydig cells (LCs) of GR1 and GR2 showed strong immunostaining of aromatase and cP450(scc) but weak staining of ER beta and AR. Interstitial cells (ICs) and Sertoli cells (SCs) expressed ER beta, particularly in GR1 and GR2. Strong expression of AR was found in peritubular cells (PCs). For all markers, expression in GR3 was the weakest. In germ cells (GCs), i.e. gonocytes and spermatogonia, aromatase and ER beta were immunoexpressed strongly whereas no expression of ERa, AR, or cP450scc was detected. It is proposed that in newborn and infantile testis, testosterone acting on PCs might modulate infant LC differentiation, whereas the absence of AR in SCs prevents development of spermatogenesis. The role of estrogen is less clear, but it could modulate the preservation of an adequate pool of precursor LCs and GCs.