Small Peptides Switch the Transcriptional Activity of Shavenbaby During Drosophila Embryogenesis
Small Peptides Switch the Transcriptional Activity of Shavenbaby During Drosophila Embryogenesis
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DOI：
10.1126/science.1188158
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发表时间：
2010-07-16
期刊：
影响因子：
56.9
通讯作者：
Kageyama, Y.
中科院分区：
文献类型：
--
作者：
Kondo, T.;Plaza, S.;Kageyama, Y.
大量真核转录物被认为是非编码rna，因为它们只包含短的开放阅读框（sorf）。然而，最近的研究结果表明，一些sorf编码小的生物活性肽。在这里，我们发现精米sORF基因编码的11到32个氨基酸的肽通过修饰转录因子Shavenbaby （Svb）来控制果蝇的表皮分化。Pri肽触发Svb蛋白的氨基末端截断，将Svb从抑制因子转化为激活因子。我们的研究结果表明，在果蝇胚胎发生过程中，Pri sORF肽对表皮形态发生的转录程序提供了严格的时间控制。
A substantial proportion of eukaryotic transcripts are considered to be noncoding RNAs because they contain only short open reading frames (sORFs). Recent findings suggest, however, that some sORFs encode small bioactive peptides. Here, we show that peptides of 11 to 32 amino acids encoded by the polished rice (pri) sORF gene control epidermal differentiation in Drosophila by modifying the transcription factor Shavenbaby (Svb). Pri peptides trigger the amino-terminal truncation of the Svb protein, which converts Svb from a repressor to an activator. Our results demonstrate that during Drosophila embryogenesis, Pri sORF peptides provide a strict temporal control to the transcriptional program of epidermal morphogenesis.