Fate of polycyclic aromatic hydrocarbons (PAH) in the rhizosphere and mycorrhizosphere of ryegrass
Fate of polycyclic aromatic hydrocarbons (PAH) in the rhizosphere and mycorrhizosphere of ryegrass
复制标题
DOI：
10.1023/a:1026587418611
复制
发表时间：
2000-01-01
期刊：
影响因子：
4.9
通讯作者：
Leyval, C
中科院分区：
文献类型：
--
作者：
Binet, P;Portal, JM;Leyval, C
多环芳烃(PAH)可以在根际降解，但也可以通过在植物组织中积累或在根表面吸附而与植被相互作用。先前的研究表明，丛枝菌根(AM)真菌有助于在多环芳烃污染的土壤中建立和维持植物。我们研究了多环芳烃在根际和菌根圈中的去向，包括生物降解、吸收和吸附。黑麦草接种或不接种莫西球菌P2(BEG69)，在盆栽中种植，盆栽土壤中添加5g kg(-1)的菲或1g kg(-1)的8种多环芳烃混合物。从根表面、根和地上部组织以及根际土壤中提取多环芳烃，并用GC-MS进行分析。在两次试验中，根际土壤中初始可提取多环芳烃浓度的0.006-0.11‰被吸附，根组织中为0.003-0.16‰，地上部组织中为0.001‰，消散的比例为36-66%，表明根际土壤中多环芳烃的大部分消散是生物降解或生物转化所致。与非菌根植物相比，有菌根植物的根部对菲和多环芳烃的吸附较少，地上部组织浓度较低，这可能有助于解释AM真菌对多环芳烃污染土壤中植物存活的有利作用。
Polycyclic aromatic hydrocarbons (PAH) can be degraded in the rhizosphere but may also interact with vegetation by accumulation in plant tissues or adsorption on root surface. Previous studies have shown that arbuscular mycorrhizal (AM) fungi contribute to the establishment and maintenance of plants in a PAH contaminated soil. We investigated the fate of PAH in the rhizosphere and mycorrhizosphere including biodegradation, uptake and adsorption. Experiments were conducted with ryegrass inoculated or not with Glomus mosseae P2 (BEG 69) and cultivated in pots filled with soil spiked with 5 g kg(-1) of anthracene or with 1 g kg(-1) of a mixture of 8 PAH in a growth chamber. PAH were extracted from root surfaces, root and shoot tissue and rhizosphere soil and were analysed by GC-MS. In both experiments, 0.006 - 0.11 parts per thousand of the initial extractable PAH concentration were adsorbed to roots, 0.003 - 0.16 parts per thousand were found in root tissue, 0.001 parts per thousand in shoot tissue and 36 - 66% were dissipated, suggesting that the major part of PAH dissipation in rhizosphere soil was due to biodegradation or biotransformation. With mycorrhizal plants, anthracene and PAH were less adsorbed to roots and shoot tissue concentrations were lower than with non mycorrhizal plants, which could contribute to explain the beneficial effect of AM fungi on plant survival in PAH contaminated soils.