A flow system for partial automation of plating efficiency tests.
A flow system for partial automation of plating efficiency tests.
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用于电镀效率测试部分自动化的流程系统。
DOI：
10.1002/cyto.990010508
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Gray,J
中科院分区：
文献类型：
--
作者：
Macevicz,S;Dean,P;Peters,D;Pinkel,D;Gray,J
已构建并评价了将已知数量的细胞无菌地沉积到收集容器中的流动系统。在该装置中，来自单细胞悬浮液的细胞流过喷嘴，该喷嘴将它们限制在出现的射流的中心。射流中的细胞由0.5 mW氦氖激光照射;它们通过激光束时散射的光进行检测、大小测定和计数。该装置已用于组织培养实验室的平板接种效率测试。我们发现，它节省了大量的劳动力，从标准的平板效率程序中删除稀释步骤。通过比较手工接种和机器接种建立的KHT、CHO‐T5和CHO‐ AA 8细胞的接种效率，确定机器接种的生物学效应。结果表明，机器接种降低了CHO-T5细胞的接种效率，而CHO-AA 8和KHT细胞的接种效率不受影响。
A flow system that sterilely deposits a known number of cells into a collection vessel has been constructed and evaluated. In this device, the cells from a single‐cell suspension flow through a nozzle that constrains them to the center of the emerging jet. The cells in the jet are illuminated by a 0.5‐mW helium‐neon laser; they are detected, sized and counted on the basis of the light scattered as they pass through the laser beam. The device has been used for plating efficiency tests in a tissue culture laboratory. We found that it saved considerable labor by eliminating the dilution step from the standard plating efficiency procedure. The biologic effect of machine plating was determined by comparing plating efficiencies of KHT, CHO‐T5, and CHO‐AA8 cells established by hand plating with those established by machine plating. Results indicate that machine plating reduces the plating efficiency of the CHO‐T5 cells while the plating efficiencies of the CHO‐AA8 and KHT cells are unaffected.