The Immunogenicity of OMP31 Peptides and Its Protection Against Brucella melitensis Infection in Mice
The Immunogenicity of OMP31 Peptides and Its Protection Against Brucella melitensis Infection in Mice
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DOI：
10.1038/s41598-019-40084-w
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发表时间：
2019-03-05
影响因子：
4.6
通讯作者：
Ding, Jianbing
中科院分区：
文献类型：
--
作者：
Zhang, Fengbo;Li, Zhiwei;Ding, Jianbing
鉴于布鲁氏菌病是一种在世界上广泛传播的人畜共患病，迫切需要一种安全有效的疫苗。最近疫苗设计的趋势已经转向基于表位的安全和特异的疫苗。本研究合成了含有OMP31 T细胞和B细胞表位的多肽，并将其应用于小鼠鼻内免疫。并对其防护效果进行评价。用OMP31的B表位或TB表位多肽免疫的小鼠血清中的IgG1和IgG2a水平较高。而用含OMP31 T细胞表位或TB表位的多肽免疫的BALB/c小鼠，在呼吸道引流淋巴结和脾的淋巴细胞中显示出高水平的产生干扰素-γ的T细胞。用羊布鲁氏菌(16M株)5×10(5)CFU滴鼻免疫小鼠后，肺内细菌载量明显低于对照组。这些数据首次证明了含有T表位、B表位或TB表位的OMP31多肽具有较高的免疫原性，从而保护宿主免受布鲁氏菌感染。
Given brucellosis is a widespread zoonosis in the world, a safe and effective vaccine is urgently needed. Recent trend in vaccine design has shifted to epitope- based vaccines that are safe and specific. In this study, peptide containing both T-cell and B-cell epitopes of OMP31 was synthesized and used to immunize the mice by nasal administration. The protective efficacy was evaluated. Mice immunized with the B epitope or TB epitope peptides of OMP31 had higher levels of IgG1 and IgG2a in the serum. While the BALB/c mice immunized with peptides containing T cell epitope or TB epitope of OMP31 showed high degree of IFN-gamma-producing T cells in the lymphocytes from the respiratory draining lymph nodes and spleen. After intranasally challenged with 5x10(5)CFU of Brucella melitensis (strain 16 M), the bacterial loads in lung of the immunized mice were significantly lower than control group. These data demonstrate for the first time that peptides of OMP31 containing T epitope, B epitope or TB epitopes are of high immunogenicity and thus can protect host from Brucella melitensis infection in lung.