Quantitative imaging of radial oxygen loss from Valisneria spiralis roots with a fluorescent planar optode
Quantitative imaging of radial oxygen loss from Valisneria spiralis roots with a fluorescent planar optode
复制标题
使用荧光平面光极对苦草根部径向氧损失进行定量成像
DOI：
10.1016/j.scitotenv.2016.06.198
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发表时间：
2016-11-01
影响因子：
9.8
通讯作者：
Xie, Xianchuan
中科院分区：
文献类型：
--
作者：
Han, Chao;Ren, Jinghua;Xie, Xianchuan
氧气（O-2）的沉积物-根界面内的可用性是至关重要的，以生存的大型植物在O-2缺乏沉积物，然而，我们的知识的细尺度的影响，大型植物根的时空动态O-2是相对有限的。在这项研究中，一个非侵入性的成像技术，利用地图周围的O-2的微分布。在36天的时间内收集的长期成像结果显示丰富的O-2时空模式范围从0到250 μ mol L-1。根诱导的O-2泄漏和随之而来的充氧面积在附近的基底根相比，发现在根尖。O-2图像显示，旋毛虫表现出径向O-2损失（ROL）沿着整个根，和O-2分布沿着根长表现出高度的小尺度的空间异质性从80%在基部的根表面下降到10%在根尖。在0 ~ 216 rimol photons m（-2）s（-1）范围内，随着根系生长和光照强度的增加，根系周围的氧合区面积随着O-2水平的增加而增加。
Oxygen (O-2) availability within the sediment-root interface is critical to the survival of macrophytes in O-2-deficient sediment; however, our knowledge of the fine-scale impact of macrophyte roots upon the spatiotemporal dynamics of O-2 is relatively limited. In this study, a non-invasive imaging technology was utilized to map O-2 micro-distribution around Valli.sneria spiralis. Long-term imaging results gathered during a 36 day-period revealed an abundance of O-2 spatiotemporal patterns ranging from 0 to 250 mu mol L-1. The root-induced O-2 leakage and consequent oxygenated area were stronger in the vicinity of the basal root compared to that found in the root tip. The O-2 images revealed V. spiralis exhibited radial O-2 loss (ROL) along the entire root, and the O-2 distribution along the root length showed a high degree of small-scale spatial heterogeneity decreasing from 80% at the basal root surface to 10% at the root tip. The oxygenated zone area around the roots increased as O-2 levels increased with root growth and irradiance intensities ranging from 0 to 216 rimol photons m(-2) s(-1), A weak ROL measuring