Effect of temporary tracheal occlusion on the endothelin system in experimental cases of diaphragmatic hernia
Effect of temporary tracheal occlusion on the endothelin system in experimental cases of diaphragmatic hernia
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DOI：
10.1080/019021490927079
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发表时间：
2005-05-01
影响因子：
1.7
通讯作者：
Laberge, JM
中科院分区：
文献类型：
--
作者：
Cloutier, M;Seaborn, T;Laberge, JM
之前，作者已经表明，气管闭塞（TO）部分逆转了先天性疝（CDH）诱导的肺动脉高压（PH）和异常肺血管发育的发作，而闭塞（TR）的释放则消除了这些临床获益。由于它们的促有丝分裂和血管活性特性，作者假设CDH羔羊肺中内皮素（ET）-1和ET受体（ETA）基因的表达增加，并且这种增加在CDH + TO中部分消除，但在CDH + TO中没有。+ TR。在妊娠80天（gd）时，在胎羊中创建手术左侧CDH，然后在108 gd时创建TO，并在129 gd时创建TR。比较了四组：CDH、CDH + TO、CDH + TO + TR和未手术对照组（C）。北方杂交检测CDH组ET-1和ETA水平明显低于CDH + TO +/- TR组（P <0.05）。除内皮细胞外，CDH +/- TO +/- TR组的气道和血管内皮素蛋白表达水平低于对照组（P <0.05）。相反，与对照组相比，CDH +/- TO +/- TR组气道和血管平滑肌的ETA蛋白表达水平更高（P <0.05）。这些结果表明，内皮素系统参与的肺动脉高压与CDH是有限的。然而，内皮素系统似乎是调制过程中的发展。
Previously, the authors have shown that tracheal occlusion ( TO) partially reverses the onset of congenital diaphragmatic hernia (CDH)-induced pulmonary hypertension (PH) and abnormal pulmonary vascular development whereas release of the occlusion (TR) abolishes these clinical benefits. As a consequence of their mitogenic and vasoactive properties, the authors hypothesize that the expression of endothelin (ET)-1 and ET receptor (ETA) genes is increased in lungs of CDH lambs, and that this increase is abolished partially in CDH + TO but not in CDH + TO + TR. A surgical left-sided CDH was created in fetal lambs at 80 days of gestation (gd), followed by TO at 108 gd, and by TR at 129 gd. Four groups were compared: CDH, CDH + TO, CDH + TO + TR, and nonoperated controls ( C). Assessment of mRNA expression by Northern blot showed significantly lower ET-1 and ETA levels in the CDH group than in the CDH + TO +/- TR groups ( P < .05). Endothelin protein expression levels were lower in CDH +/- TO +/- TR groups when compared with controls for airways and vessels ( P < .05) with the exception of endothelial cells. In contrast, ETA protein expression levels were higher in CDH +/- TO +/- TR groups compared with controls for airways and blood vessels smooth muscles ( P < .05). These results suggest that involvement of the endothelin system in the pulmonary hypertension associated with CDH is limited. However, the endothelin system appears to be modulated during development.