Construction of a potato fraction library for the investigation of functional secondary metabolites
Construction of a potato fraction library for the investigation of functional secondary metabolites
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用于研究功能性次生代谢物的马铃薯组分库的构建
DOI：
10.1080/09168451.2018.1525273
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Osada H.
中科院分区：
文献类型：
--
作者：
Nogawa T;Futamura Y;Okano A;Suto M;Nakamura J;Ishihara K;Osada H.
以马铃薯为研究对象，建立了北川、皮尔卡、樱花、大西洋、丰shiro、斯诺登、Kitamurasaki和Northern Ruby 8个马铃薯品种的功能性次生代谢产物文库，将其分为花、叶、茎、根、块茎皮和块茎。每个馏分都是半纯化的萃取物，约有800个馏分用于文库。采用DAD-LC/MS分析获得结构信息，并对其进行各种生物活性评价。LC/MS数据表明，主成分分析（PCA）支持了每个部分的特定特征。在30 μg/mL浓度下，约40%的提取物具有显著的生物活性，其中花提取物具有较强的细胞毒性。活性分析和质谱分析结果表明，马铃薯花具有较强的细胞毒性，可能来源于马铃薯糖生物碱的复杂混合物。此外，块茎皮提取物还显示出较强的抗疟活性，这在以前的研究中没有报道过。部分提取物具有明显的抑菌活性。
A potato fraction library was constructed to investigate functional secondary metabolites from 8 cultivars: Kitahime, Pilka, Sakurafubuki, Atlantic, Toyoshiro, Snowden, Kitamurasaki, and Northern Ruby, which were divided into flower, leaf, stem, roots, tuber peel, and tuber. Each fraction was a semi-purified extract and about 800 fractions were prepared for the library. They were analyzed by DAD-LC/MS to obtain structural information and were evaluated for various biological activities. LC/MS data showed that each part had a specific characteristic for their constituents supported by principal component analysis (PCA). Approximately 40% of fractions showed significant biological activities at 30 μg/mL, especially the flower fractions showed strong cytotoxicity. PCAs based on the activity and LC/MS data suggested that the strong cytotoxicity of flowers was derived from a complex mixture of potato glycoalkaloids. In addition, tuber peel fractions showed strong antimalarial activity, which had not been reported before. Also, some fractions showed significant antibacterial activities.