Mitochondrial fusion in Chlamydomonas reinhardtii zygotes.
Mitochondrial fusion in Chlamydomonas reinhardtii zygotes.
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DOI：
10.1016/j.ejcb.2012.10.004
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发表时间：
2013-02
影响因子：
6.6
通讯作者：
Dirk Scholz;B. Westermann
中科院分区：
文献类型：
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作者：
Dirk Scholz;B. Westermann
线粒体是动态细胞器，被发现在许多不同的细胞类型中融合和分裂。线粒体融合在呼吸能力的维持、代谢能量的耗散和线粒体DNA的遗传中发挥着重要作用。虽然酵母和哺乳动物中线粒体融合的分子机制已被详细描述，但对高等植物和藻类中的线粒体融合知之甚少。我们询问是否可以在单细胞绿藻莱茵衣藻中观察到线粒体融合。用荧光染料对配子中的线粒体进行染色，并通过荧光显微镜检测受精卵中荧光标记的混合，表明融合。在野生型受精卵和呼吸突变体中观察到线粒体融合，尽管效率较低。我们得出结论，线粒体很容易在绿藻中融合。
Mitochondria are dynamic organelles that were found to fuse and divide in many different cell types. Mitochondrial fusion plays important roles in maintenance of respiratory capacity, dissipation of metabolic energy, and inheritance of mitochondrial DNA. While the molecular machinery of mitochondrial fusion has been characterized in great detail in yeast and mammals, only little is known about mitochondrial fusion in higher plants and algae. We asked whether mitochondrial fusion can be observed in the unicellular green alga Chlamydomonas reinhardtii. Mitochondria were stained with fluorescent dyes in gametes, and mixing of fluorescent markers was detected by fluorescence microscopy in zygotes indicating fusion. Mitochondrial fusion was observed in wild type zygotes, and also in respiratory mutants, albeit with less efficiency. We conclude that mitochondria readily fuse in green algae.