Lipid nanoparticles for brain targeting III. Long-term stability and in vivo toxicity
Lipid nanoparticles for brain targeting III. Long-term stability and in vivo toxicity
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DOI：
10.1016/j.ijpharm.2013.06.037
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发表时间：
2013-09-15
影响因子：
5.8
通讯作者：
Ricci, Maurizio
中科院分区：
文献类型：
--
作者：
Blasi, Paolo;Schoubben, Aurelie;Ricci, Maurizio
用途：这项工作的目的是评估的长期稳定性和安全性的脂质纳米粒用于脑drugdelivery.Methods：脂质纳米粒，制备高压均质化，储存在室温和4摄氏度，并监测其平均流体动力学直径和高斯分布宽度随时间的推移。十六烷基棕榈酸酯和聚山梨酯（R）80的化学完整性通过核磁共振诊断信号进行了研究。纳米颗粒的毒性进行了评估，在鸡胚绒毛尿囊膜试验和啮齿动物，脑组织学evaluation.Results：数据显示，在4摄氏度的纳米颗粒的稳定性超过一段时间的4年，只有一个有限的粒径增加，而在室温下不稳定，观察后9个月。核磁共振研究证实了（
Purpose: The aim of the work was to assess the long-term stability and the safety of lipid nanoparticles intended for brain drug delivery.Methods: Lipid nanoparticles, prepared by high pressure homogenization, were stored at room temperature and 4 degrees C and monitored for their mean hydrodynamic diameter and Gaussian distribution width over time. Cetylpalmitate and polysorbate (R) 80 chemical integrity were investigated by nuclear magnetic resonance on diagnostic signals. Nanoparticle toxicity was assessed in chicken embryos by chorioallantoic membrane assay and in rodents,by brain histological evaluation.Results: Data showed nanoparticle stability at 4 degrees C over a period of time of 4 years with only a limited particle size increase while at room temperature destabilization was observed after 9 months. Nuclear magnetic resonance investigation confirmed the absence (