Integrated analytical techniques with high sensitivity for studying brain translocation and potential impairment induced by intranasally instilled copper nanoparticles
Integrated analytical techniques with high sensitivity for studying brain translocation and potential impairment induced by intranasally instilled copper nanoparticles
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DOI：
10.1016/j.toxlet.2014.01.041
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发表时间：
2014-04-07
影响因子：
3.5
通讯作者：
Chen, Chunying
中科院分区：
文献类型：
--
作者：
Bai, Ru;Zhang, Lili;Chen, Chunying
吸入暴露于工程纳米材料的健康影响已引起越来越多的关注。本文利用高灵敏度的综合分析技术，研究了经鼻滴入纳米铜(CuNPs)引起的脑移位和潜在损伤。小鼠暴露于不同剂量(1、10、40 mg/kgbw)的CuNPs。10和40 mg/kg剂量组小鼠体重明显下降(p<；0.05)，但在染毒持续时间内略有恢复。电感耦合等离子体质谱(ICPMS)分析表明，CuNPs可以进入大脑。用同步辐射X射线荧光(SRXRF)观察到一些重要金属元素的分布发生了变化。H&E染色和免疫组织化学分析表明，CuNPs对神经细胞有损伤作用，星形胶质细胞可能是CuNPs的潜在靶点之一。不同脑区神经递质水平的变化表明，接触组存在功能障碍。这些数据表明，CuNPs经鼻腔吸入后可进入大脑，并对中枢神经系统(CNS)造成损害。集成有效的分析技术进行系统研究是更好地了解纳米材料的生物活性的一个有前途的方向。(C)2014爱思唯尔爱尔兰有限公司。保留所有权利。
Health impacts of inhalation exposure to engineered nanomaterials have attracted increasing attention. In this paper, integrated analytical techniques with high sensitivity were used to study the brain translocation and potential impairment induced by intranasally instilled copper nanoparticles (CuNPs). Mice were exposed to CuNPs in three doses (1, 10, 40 mg/kg bw). The body weight of mice decreased significantly in the 10 and 40 mg/kg group (p < 0.05) but recovered slightly within exposure duration. Inductively coupled plasma mass spectrometry (ICP-MS) analysis showed that CuNPs could enter the brain. Altered distribution of some important metal elements was observed by synchrotron radiation X-ray fluorescence (SRXRF). H&E staining and immunohistochemical analysis showed that CuNPs produced damages to nerve cells and astrocyte might be the one of the potential targets of CuNPs. The changes of neurotransmitter levels in different brain regions demonstrate that the dysfunction occurred in exposed groups. These data indicated that CuNPs could enter the brain after nasal inhalation and induced damages to the central nervous system (CNS). Integration of effective analytical techniques for systematic investigations is a promising direction to better understand the biological activities of nanomaterials. (C) 2014 Elsevier Ireland Ltd. All rights reserved.