Cellular localization of Arabidopsis xyloglucan endotransglycosylase-related proteins during development and after wind stimulation
Cellular localization of Arabidopsis xyloglucan endotransglycosylase-related proteins during development and after wind stimulation
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DOI：
10.1104/pp.115.4.1319
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发表时间：
1997-12-01
期刊：
影响因子：
7.4
通讯作者：
Braam, J
中科院分区：
文献类型：
--
作者：
Antosiewicz, DM;Purugganan, MM;Braam, J
拟南芥中存在一个编码木葡聚糖内糖基转移酶（XET）相关蛋白的基因家族。TCH4是该家族的成员，受环境刺激强烈上调，并编码能够修饰细胞壁木葡聚糖的XET。为了研究XET定位，我们产生了针对TCH4羧基末端的抗体。这些抗体识别TCH4和可能的其他XET相关蛋白。这些数据表明，叶黄素积累在扩大的细胞，在网站的细胞间隙的空气形成，并在基础的叶，子叶和下胚轴。黄原胶也积累在维管组织中，在那里细胞壁的改变导致了管分子和筛管的形成。因此，Xeplatin可以在修饰细胞壁以允许生长、气隙形成、脉管系统发育和在机械应变下的区域的增强中起作用。风刺激后，整体XET水平出现下降风刺激植物的叶片。然而，与风后TCH4 mRNA水平的增加一致，有一些区域显示出增加的免疫反应，包括髓实质细胞周围的网站，维管元件之间，和表皮内。这些结果表明，TCH4可能有助于适应性变化的形态发生在拟南芥暴露于机械刺激。
A gene family encoding xyloglucan endotransglycosylase (XET)-related proteins exists in Arabidopsis. TCH4, a member of this family, is strongly up-regulated by environmental stimuli and encodes an XET capable of modifying cell wall xyloglucans. To investigate XET localization we generated antibodies against the TCH4 carboxyl terminus. The antibodies recognized TCH4 and possibly other XET-related proteins. These data indicate that XETs accumulate in expanding cells, at the sites of intercellular airspace formation, and at the bases of leaves, cotyledons, and hypocotyls. XETs also accumulated in vascular tissue, where cell wall modifications lead to the formation of tracheary elements and sieve tubes. Thus, XETs may function in modifying cell walls to allow growth, airspace formation, the development of vasculature, and reinforcement of regions under mechanical strain. Following wind stimulation, overall XET levels appeared to decrease in the leaves of wind-stimulated plants. However, consistent with an increase in TCH4 mRNA levels following wind, there were regions that showed increased immunoreaction, including sites around cells of the pith parenchyma, between the vascular elements, and within the epidermis. These results indicate that TCH4 may contribute to the adaptive changes in morphogenesis that occur in Arabidopsis following exposure to mechanical stimuli.