Guinea-pig-derived allergens. Clinicoimmunologic studies, characterization, airborne quantitation, and size distribution.
Guinea-pig-derived allergens. Clinicoimmunologic studies, characterization, airborne quantitation, and size distribution.
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豚鼠衍生的过敏原。
DOI：
10.1164/arrd.1984.129.5.844
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Reed,CE
中科院分区：
文献类型：
--
作者：
Swanson,MC;Agarwal,MK;Yunginger,JW;Reed,CE
通过放射性过敏吸附试验 (RAST)，在接触实验动物后出现过敏症状的实验室工作人员的 20 份血清样本中，9 份含有针对豚鼠尿液、毛皮或白蛋白的 IgE 抗体升高。皮肤测试记录了所有 3 种豚鼠过敏原的过敏活性。在 RAST 抑制测定中研究了这 3 种豚鼠过敏原的交叉反应性。豚鼠毛皮提取物和尿液在所有 3 项测定中均产生剂量相关的抑制，但豚鼠白蛋白仅在同源测定中具有抑制作用。通过凝胶过滤、聚丙烯酰胺凝胶电泳和等电聚焦的表征证明了过敏原的异质性。使用大容量空气采样器在豚鼠饲养室、研究实验室和图书馆中采集空气过滤器样本。还采集了室外空气过滤器样本。通过 RAST 抑制，在所有室内空气过滤器样品中检测到豚鼠尿液过敏原活性。除图书馆和室外外，所有空气过滤器样本均检测到豚鼠毛皮过敏原活性。在任何空气过滤器样本中均未检测到豚鼠白蛋白。空气中颗粒的大小表明，大多数豚鼠过敏原活性与直径大于 5μm 和小于 0.8μm 的颗粒有关。尿液似乎是豚鼠过敏原的主要来源，它存在于空气中的颗粒中，小到足以在吸入时穿透下呼吸道。
Of 20 serum samples from laboratory workers who experienced allergic symptoms after exposure to laboratory animals, 9 contained elevated IgE antibodies to guinea pig urine, pelt, or albumin by the radioallergosorbent test (RAST). Skin testing documented allergenic activity with all 3 guinea pig allergens. The cross-reactivity of these 3 guinea pig allergens was studied in RAST inhibition assays. Guinea pig pelt extract and urine produced dose-related inhibition in all 3 assays, but guinea pig albumin was inhibitory in only the homologous assay. Characterization by gel filtration, Polyacrylamide gel electrophoresis, and isoelectric focusing demonstrated heterogeneity of the allergens. Air filter samples were taken in a guinea pig housing room, a research laboratory, and a library, using a high volume air sampler. An outdoor air filter sample was also taken. Guinea pig urine allergen activity was detected in all indoor air filter samples by RAST inhibition. Guinea pig pelt allergen activity was detected in all air filter samples, except the library and outdoors. Guinea pig albumin was not detected in any air filter sample. Sizing of airborne particles demonstrated that most guinea pig allergen activity was associated with particles of a diameter greater than 5µand less than 0.8µ.Urine appears to be the major source of guinea pig allergens, and it is present in airborne particles small enough to penetrate the lower respiratory tract when inhaled.