Involvement of the S-layer proteins Hpi and SlpA in the maintenance of cell envelope integrity in Deinococcus radiodurans R1
Involvement of the S-layer proteins Hpi and SlpA in the maintenance of cell envelope integrity in Deinococcus radiodurans R1
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DOI：
10.1099/mic.0.28971-0
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发表时间：
2006-09-01
期刊：
影响因子：
2.8
通讯作者：
Lidstrom, Mary E.
中科院分区：
文献类型：
--
作者：
Rothfuss, Heather;Lara, Jimmie C.;Lidstrom, Mary E.
已调查的潜在功能的两个蛋白质在Deinococcus radiodurans R1预测参与的维护和完整的S层：六角包装的中间（HPI）蛋白，和SlpA（DR 2577），一个同源物的S-层SlpA蛋白在嗜热栖热菌。hpi基因的缺失对细胞被膜的结构或剪切或溶剂诱导的应力反应几乎没有影响。然而，slpA基因的缺失引起细胞包膜结构的实质性改变，以及与野生型相比对溶剂和剪切应力的抗性的显著缺陷。slpA突变细胞的超微结构分析表明，大部分的外部Hpi蛋白碳水化合物外壳，“粉红色的外套”，和膜样背衬层的损失。这些结果表明SlpA蛋白可能参与了Hpi表面层与细胞内被膜的粘附，并且SlpA可能在维持D.耐辐射的
The potential functions have been investigated of two proteins in Deinococcus radiodurans R1 predicted to be involved in the maintenance and integrity of the S layer: the hexagonally packed intermediate (Hpi) protein, and SlpA(DR2577), a homologue of an S-layer SlpA protein in Thermus thermophilus. Deletion of the hpi gene had little effect on the structure of the cell envelope or on shear- or solvent-induced stress responses. However, deletion of the slpA gene caused substantial alterations in cell envelope structure, and a significant defect in resistance to solvent and shear stresses compared to the wild-type. Ultrastructural analysis of slpA mutant cells indicated loss of much of the outer Hpi protein carbohydrate coat, the 'pink envelope', and the membrane-like backing layer. Together these results suggest that the SlpA protein may be involved in attachment of the Hpi surface layer to the inner cell envelope, and that SlpA may play an important role in the maintenance of cell envelope integrity in D. radiodurans.