C-FOS EXPRESSION IN RAT LUMBAR SPINAL-CORD FOLLOWING PERIPHERAL STIMULATION IN ADJUVANT-INDUCED ARTHRITIC AND NORMAL RATS
C-FOS EXPRESSION IN RAT LUMBAR SPINAL-CORD FOLLOWING PERIPHERAL STIMULATION IN ADJUVANT-INDUCED ARTHRITIC AND NORMAL RATS
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DOI：
10.1016/0006-8993(93)91507-o
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发表时间：
1993-04-02
期刊：
影响因子：
2.9
通讯作者：
BESSON, JM
中科院分区：
文献类型：
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作者：
ABBADIE, C;BESSON, JM
我们以前的数据报道，在弗氏佐剂注射三周后，c-fos即刻早期基因在非刺激性多发性关节炎大鼠神经元的腰髓中表达最高。本研究利用c-fos表达来判断正常大鼠和氯胺酮麻醉下的关节炎大鼠脊髓神经元对施加于踝关节的机械压力的反应性。结果表明，氯胺酮麻醉非刺激性关节炎大鼠腰椎各节段Fos免疫反应阳性神经元数目(1)略低于非刺激性非刺激性关节炎大鼠，(2)刺激性正常和关节炎动物均显著高于非刺激性动物，尤其是L3、L4的I、II、V、VI板层；(3)刺激性关节炎大鼠腰椎节段各节段的Fos免疫反应阳性神经元数目均显著高于刺激性正常大鼠。佐剂性关节炎大鼠基础Fos标记的出现和Fos样免疫反应神经元数量的增加，间接提示这些神经元异常活跃，参与了关节炎的痛敏反应。因此，在慢性疼痛模型中使用Fos样免疫反应似乎是研究各种药理化合物，如止痛药和抗炎药的可能作用的合适工具。
Our previous data reported a maximal expression of the c-fos immediate-early gene in the lumbar spinal cord of the non-stimulated polyarthritic rat neurons, three weeks after Freund's adjuvant injection. The present study utilises c-fos expression to judge the reactivity of spinal neurons to calibrated mechanical pressure applied to the ankle joint, in both normal and arthritic rats under ketamine anesthesia. The results indicate that the number of Fos-like immunoreactive neurons (1) is slightly decreased in ketamine-anesthetized non-stimulated arthritic rats as compared to the non-anesthetized non-stimulated ones, (2) is significantly higher in both stimulated normal and arthritic animals as compared to non-stimulated animals, particularly in laminae I, II, V and VI of L3 and L4, and (3) is significantly increased in stimulated arthritic as compared to stimulated normal rats, in all laminae of lumbar spinal segments. The appearance of 'basal' Fos labeling during the adjuvant-induced arthritic disease and the increased number of Fos-like immunoreactive neurons in stimulated arthritic rats compared to stimulated normal animals indirectly suggests that these neurons are abnormally active and thus involved in the hyperalgesia of arthritic disease. Therefore the use of Fos-like immunoreactivity in the chronic pain model seems to be an appropriate tool to study possible effects of various pharmacological compounds, such as analgesics and anti-inflammatory drugs.