Copper acquisition by the SenC protein regulates aerobic respiration in Pseudomonas aeruginosa PAO1
Copper acquisition by the SenC protein regulates aerobic respiration in Pseudomonas aeruginosa PAO1
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DOI：
10.1111/j.1574-6968.2009.01726.x
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发表时间：
2009-09-01
影响因子：
2.1
通讯作者：
Haas, Dieter
中科院分区：
文献类型：
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作者：
Frangipani, Emanuela;Haas, Dieter
铜绿假单胞菌的有氧呼吸涉及四个属于血红素-铜家族的末端氧化酶（即，三个细胞色素c氧化酶和一个醌醇氧化酶）加上一个铜非依赖性、氰化物不敏感的醌醇氧化酶（CIO）。PA 0114基因编码SCO 1/SenC型蛋白，已知其对于酵母中的铜递送至细胞色素c是重要的，红细菌属（Rhodobacter spp.）和根癌农杆菌，被认为是重要的铜收购和好氧呼吸铜绿假单胞菌。与野生型PAO 1相比，PA 0114（senC）突变体在低铜培养基中生长不良，细胞色素cbb（3）型氧化酶活性低，但CIO表达水平升高。添加铜逆转了这些表型，表明SenC蛋白对周质铜的捕获有助于铜绿假单胞菌适应铜剥夺。
Aerobic respiration of Pseudomonas aeruginosa involves four terminal oxidases belonging to the heme-copper family (that is, three cytochrome c oxidases and one quinol oxidase) plus one copper-independent, cyanide-insensitive quinol oxidase (CIO). The PA0114 gene encoding an SCO1/SenC-type protein, which is known to be important for copper delivery to cytochrome c in yeast, Rhodobacter spp. and Agrobacterium tumefaciens, was found to be important for copper acquisition and aerobic respiration in P. aeruginosa. A PA0114 (senC) mutant grew poorly in low-copper media and had low cytochrome cbb(3)-type oxidase activity, but expressed CIO at increased levels, by comparison with the wild-type PAO1. Addition of copper reversed these phenotypes, suggesting that periplasmic copper capture by the SenC protein helps P. aeruginosa to adapt to copper deprivation.