Immobilization of Cibacron blue F3GA on electrospun polysulphone ultra-fine fiber surfaces towards developing an affinity membrane for albumin adsorption
Immobilization of Cibacron blue F3GA on electrospun polysulphone ultra-fine fiber surfaces towards developing an affinity membrane for albumin adsorption
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DOI：
10.1016/j.memsci.2006.05.027
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发表时间：
2006-10-05
影响因子：
9.5
通讯作者：
Ramakrishna, Seeram
中科院分区：
文献类型：
--
作者：
Ma, Zuwei;Masaya, Kotaki;Ramakrishna, Seeram
采用静电纺丝技术制备的纤维直径为纳米或微米级的非织造布聚合物膜是一种潜在的亲和膜应用材料。本文以Cibacron Blue F3GA(CB)和牛血清白蛋白(BSA)为配体配位模型，对电纺聚砜(PSU)纤维膜进行了表面改性，并研究了其作为亲和膜的性能。以Ce(W)为引发剂，在PSU纤维表面接枝甲基丙烯酸(MAA)，引入羧基。以碳化二亚胺为偶联剂，将羧基与二氨基二丙胺(DADPA)反应生成氨基。最后，通过与氨基的反应，将CB共价连接到PSU纤维表面。用ATR-FTIR、XPS等比色方法对膜的表面进行了表征。新型PSU亲和膜比商品微孔膜具有更高的透水性。测定了牛血清白蛋白在CB固定化PSU膜上的吸附等温线。CB功能化的PSU膜对BSA的捕获量为22 mg/g。用离子强度和pH值增加的洗脱缓冲液对膜进行再生，使膜可重复使用。通过测定亲和膜的穿透曲线和洗脱曲线，了解亲和膜对BSA的动态吸附和解吸过程。这项工作证明了电纺聚合物纤维膜具有潜在的亲和膜应用前景。(C)2006爱思唯尔B.V.保留所有权利。
Non-woven polymer membrane with nano- or micro-scaled fiber diameter prepared by electrospinning technique is a potential material for affinity membrane application. In this work, electrospun polysulphone (PSU) fiber membrane was surface modified and studied as a novel affinity membrane, using Cibacron blue F3GA (CB) and bovine serum albumin (BSA) as a ligand-ligate research model. Methacrylic acid (MAA) was graft polymerized on the PSU fiber surface initiated by Ce(W) to introduce carboxyl groups. With carbodiimide as coupling agent, the carboxyl groups were reacted with diamino-dipropylamine (DADPA) to introduce amino groups. Finally, CB was covalently attached on the PSU fiber surface through reaction with the amino groups. The surface of the membrane was characterized by ATR-FTIR, XPS and other colorimetric methods. The novel PSU affinity membrane showed higher water permeability than commercial microporous membranes. BSA adsorption isotherm curve on the CB immobilized PSU membrane was tested. The CB functionalized PSU membrane showed a capturing capacity of 22 mg/g for BSA. Regeneration of the membrane using elution buffer with increased ionic strength and pH value made the membrane reusable. To understand the dynamic adsorption and desorption of BSA, breakthrough curve and elution curve of the affinity membrane were tested. This work demonstrated that electrospun polymer fiber membrane has potential for affinity membrane applications. (c) 2006 Elsevier B.V. All rights reserved.