Arenimonas soli sp. nov., isolated from saline-alkaline soil.
Arenimonas soli sp. nov., isolated from saline-alkaline soil.
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DOI：
10.1099/ijsem.0.002026
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发表时间：
2017-08
影响因子：
2.8
通讯作者：
Lian Xu;Ji-Quan Sun;Xia Liu;Xiao-zhen Liu;M. Qiao;Xiao‐Lei Wu
中科院分区：
文献类型：
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作者：
Lian Xu;Ji-Quan Sun;Xia Liu;Xiao-zhen Liu;M. Qiao;Xiao‐Lei Wu
从内蒙古北方杭锦旗盐碱地土壤中分离到一株革兰氏染色阴性的非运动好氧细菌，命名为Y3 L17 T。Y3 L17 T在15-45 °C（最适35 °C）、pH 6.0-10.0（最适pH 8.0）和0- 4%（w/v）NaCl（最适0%）下生长。基于16 S rRNA基因和gyrB基因序列的系统发育分析结果表明，Y3 L17 T与沙单胞菌属的成员紧密聚在一起，与Aestuarii S2- 21 T具有最高的16 S rRNA基因相似性（99.5%）和东海沙殖单胞菌（ArenimonasdonghaensisHO 3-R19 T）（98.2%），与该属其它模式菌株的相似性均低于97%。Y3 L17 T与A. Aestuarii S2-21T. Y3 L17 T与A. aestuariiS 2 - 21 T和A.东海藻HO 3-R19 T的表达量分别为20.1±2.5和18.2± 3.2%。Y3 L17 T含有磷脂酰乙醇胺、二磷脂酰甘油、磷脂酰甘油、5种未知磷脂和1种未知脂质作为主要极性脂质。呼吸醌主要为泛醌-8（Q-8），细胞脂肪酸主要为异C15：0、异C17：0ω 9 c和异C11：0 3-OH。其基因组DNA G+C含量为65.4mol%。根据其表型、系统发育和基因型特征，Y3 L17 T代表了Arenimonas属中的一个新种，建议将其命名为Arenimonasolisp. nov.，模式菌株为Y3 L17 T（=CGMCC 1.15905T =KCTC 52420 T）。
A Gram-staining-negative, non-motile, aerobic bacterial strain, designated Y3L17T, was isolated from the saline-alkaline soil of a farmland, Hangjin Banner, Inner Mongolia, northern China. Y3L17T could grow at 15-45 °C (optimum 35 °C), pH 6.0-10.0 (optimum pH 8.0) and with 0-4 % (w/v) NaCl (optimum 0 %). The results of phylogenetic analysis based on the 16S rRNA gene and gyrB gene sequences revealed that Y3L17T tightly clustered with strains of members of the genus Arenimonas, sharing the highest 16S rRNA gene similarities with Arenimonas aestuarii S2-21T (99.5 %) and Arenimonas donghaensis HO3-R19T (98.2 %), and lower similarities (<97 %) with all the other type strains of species of this genus. However, Y3L17T shared only 92.62 % gyrB gene similarities with A. aestuarii S2-21T. The DNA-DNA hybridization values of Y3L17T with A. aestuariiS2-21T and A. donghaensis HO3-R19T were 20.1±2.5 and 18.2±3.2 %, respectively. Y3L17T contained phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, five unknown phospholipids and one unknown lipid as the major polar lipids. Ubiquinone-8 (Q-8) was the predominant respiratory quinone, while iso-C15 : 0, iso-C17 : 0ω9c and iso-C11 : 0 3-OH were the major cellular fatty acids. Its genomic DNA G+C content was 65.4 mol%. On the basis of its phenotypic, phylogenetic and genotypic characteristics, Y3L17T represents a novel species within the genus Arenimonas, for which the name Arenimonas soli sp. nov. is proposed, the type strain is Y3L17T (=CGMCC 1.15905T =KCTC 52420T).