Cell growth optimization in microcarrier culture
Cell growth optimization in microcarrier culture
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微载体培养中的细胞生长优化
DOI：
10.1007/bf02619423
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
H. Hopps
中科院分区：
文献类型：
--
作者：
B. Mered;P. Albrecht;H. Hopps
本研究采用微载体培养技术对猴肾细胞系和鸡胚原代细胞进行了培养。载体支持物是根据马萨诸塞州理工学院开发的方案制备的低阴离子交换容量的DEAE-Sephadex凝胶珠。1.0 - 2.0 mg珠/ml组织培养基的珠浓度和20，000个细胞/cm 2珠表面的细胞接种物似乎是最佳的。微载体培养系统的效率进行了比较，固定和滚瓶培养。固定瓶的细胞密度比滚瓶的最大密度高约2倍，比微载体培养中的细胞密度高约3倍和2倍，珠浓度分别为2.5和1.0 mg/ml，就每毫升组织培养基的细胞产量而言，微载体培养上级优于滚瓶和固定培养。微载体培养系统的优点是其适合于按比例放大到大体积生产单元。
SummaryThree monkey kidney cell lines and primary chicken embryo cells were grown in microcarrier culture. The carrier support was DEAE-Sephadex gel beads at low anion exchange capacity prepared according to a protocol developed at the Massachusetts Institute of Technology.The growth rate of the cells and the final cell density in microcarrier culture was dependent on the concentration of the beads in culture and on the size of the initial cell inoculum. A bead concentration of 1.0 to 2.0 mg of beads/ml of tissue culture medium and a cell inoculum of 20,000 cells/cm2 of bead surface appeared to be optimal. The efficiency of the microcarrier culture system was compared to that of stationary and roller bottle cultures. Stationary flasks gave cell densities about twofold higher than maximal densities in roller bottles and about threefold and twofold higher than cell densities in microcarrier culture at a bead concentration of 2.5 and 1.0 mg/ml, respectively.In terms of cell yield per millitier of tissue culture medium, the microcarrier culture was superior to roller bottle and stationary cultures. An advantage of the microcarrier culture system is its suitability for a scale up into large volume production units.