Broad-spectrum sun-protective action of Porphyra-334 derived from Porphyra vietnamensis.
Broad-spectrum sun-protective action of Porphyra-334 derived from Porphyra vietnamensis.
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DOI：
10.4103/0974-8490.60578
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发表时间：
2010-01
影响因子：
0.7
通讯作者：
Nanda S
中科院分区：
文献类型：
--
作者：
Bhatia S;Sharma K;Namdeo AG;Chaugule BB;Kavale M;Nanda S
目前世界市场上有大量的紫外线防护化合物，其中98%可以防护UV-B范围，其余2%仅能有效防护远紫外线A范围。此外，这些合成化合物具有与光稳定性和交叉稳定性相关的各种问题。对于防晒剂的需求是至关重要的，该防晒剂将在长时间内保持稳定，并提供针对有害紫外线范围的广谱保护。印度洋含有大量的大型藻类，它们通过什克酸途径合成不同数量的真菌孢子素氨基酸，“防晒化合物”。在本研究中，我们评估了紫菜-334提供的防晒保护，紫菜-334是一种从印度紫菜中分离出的菌孢素氨基酸。此外，所分离的化合物通过高效薄层色谱（HPTLC）指纹图谱，高效液相色谱（HPLC）和紫外（UV）检测，而核磁共振（NMR）光谱和红外光谱用于其结构表征。在不同储存和pH条件下进行稳定性研究。最后，防晒配方的开发和其潜力，对市售芦荟凝胶进行了评估，在体外防晒保护方法。据观察，紫菜-334的防晒潜力是市售芦荟凝胶制剂的5.11倍。
There are enormous UV-protective compounds present in the current world market, out of which 98% give protection against UV-B range and the remaining 2% are potent against far UV-A range only. Furthermore, these synthetic compounds have various problems related to photo-stability and cross-stability. There is a vital need of sunscreen agents that will remain stable for prolonged periods and provide broad-spectrum protection against harmful UV range. The Indian Ocean contains large amounts of macro-algae which synthesize varied amount of mycosporine amino acids, “sun-protective compounds” by shikmic acid pathway. In the present study, we have evaluated the sunscreen protection provided by Porphyra-334, a mycosporine amino acid isolated from Indian sp. of Porphyra. Furthermore, the isolated compound was detected by high performance thin layer chromatography (HPTLC) fingerprinting, high performance liquid chromatography (HPLC) and ultraviolet (UV), whereas nuclear magnetic resonance (NMR) spectroscopy and infrared spectrometry were used for its structural characterization. Stability studies were performed under different storage and pH conditions. Ultimately a sunscreen formulation was developed and its potential against marketed Aloe vera gel was evaluated by in vitro sunscreen protection method. It was observed that sunscreen potential of Porphyra-334 was 5.11-fold greater than that of the marketed Aloe vera gel preparation.