Large indentation method to measure elasticity of cell in robot-integrated microfluidic chip
Large indentation method to measure elasticity of cell in robot-integrated microfluidic chip
复制标题
机器人集成微流控芯片中细胞弹性的大压痕法测量
DOI：
10.1109/lra.2017.2717500
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Fumihito Arai
中科院分区：
文献类型：
--
作者：
Hirotaka Sugiura;Shinya Sakuma;Makoto Kaneko;Fumihito Arai
机器人集成微流控芯片是实现高通量、高精度单细胞力学特性分析的有效工具。微流控芯片中使用了一对机械微探针来测量细胞的变形和反作用力。在我们以前的研究中，我们在这个测量系统中测量了细胞的弹性。但是，这种测量方法局限于细胞的小变形区域，存在一些问题。为了避免这些问题，我们改变了测量方法，利用细胞的大变形区域。首先，我们提出一种微分式取样云纹法，以扩大力或位移量测的范围。其次，改进了单胞的力学模型，使其能够表达大变形区域的变形特性。变形模型由高分子理论推导。因此，测得的弹性与细胞内细丝网络有关。结果，我们完成了在一个大的变形区域内使用的实验数据测量的细胞的弹性。本研究为测量细胞弹性提供了一种更为实用、可靠的方法。
Robot-integrated microfluidic chip is a promising tool to realize mechanical characterization of a single cell with high throughput and high accuracy. The microfluidic chip used in our system has a pair of mechanical microprobes to measure deformation and reaction force of cells. In our previous studies, we measured the elasticity of cells in this measurement system. However, there were some problems on the measurement method limited in small deformation regions of cell. To avoid these problems, we changed the measurement method to utilize large deformation regions of cell. First, we proposed a differential-type sampling moiré method to extend the range of the force or displacement measurement. Second, the mechanical model of the cell was improved to express the deformation characteristics up to large deformation region. The deformation model was derived from high-molecular theory. Therefore, the measured elasticity was related to the intracellular filament network. As a result, we accomplished in measuring the elasticity of the cells using the experimental data in a large deformation region. This study provided us with a more practical and reliable method to measure cellular elasticity.