LEUKOCYTE INVOLVEMENT IN CEREBRAL INFARCT GENERATION AFTER ISCHEMIA AND REPERFUSION
LEUKOCYTE INVOLVEMENT IN CEREBRAL INFARCT GENERATION AFTER ISCHEMIA AND REPERFUSION
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DOI：
10.1016/0361-9230(94)90010-8
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发表时间：
1994-01-01
影响因子：
3.8
通讯作者：
TUMA, RF
中科院分区：
文献类型：
--
作者：
HEINEL, LA;RUBIN, S;TUMA, RF
在大鼠缺血和再灌注损伤后，评价了参与脑梗塞产生的白色血细胞。在全前脑缺血模型中，在缺血1小时和再灌注1小时15分钟后，比较对照组和白细胞减少组大鼠（由长春碱诱导，白细胞计数< 1500/mm 3）。使用氯化三苯基四氮唑（TTC）染色在冠状脑切片上确定脑梗死。并对脑电图（EEG）和体感诱发电位（SSEP）进行比较。结果表明，白细胞减少大鼠的梗死面积显著小于相应对照组的梗死面积（21 +/-16% vs. 70 +/-16%）。此外，EEG保存在所有白细胞减少的动物相比，对照组，无论是在缺血和再灌注后。皮质峰成分的SSEP也更好地保存在白细胞减少动物在缺血和再灌注60分钟。这些结果表明，在全前脑缺血和再灌注模型中，白色血细胞参与了脑损伤的产生，如脑梗塞的TTC染色所示。
White blood cell involvement in the generation of cerebral infarcts was evaluated following ischemia and reperfusion injury in the rat. Control and leukopenic rats (induced by vinblastine, WBC counts < 1500/mm(3)) were compared in a global forebrain ischemic model after 1 h of ischemia and 1 h 15 min of reperfusion. Cerebral infarcts were defined on coronal brain sections using Triphenyl tetrazolium chloride (TTC) staining. Electroencephalographic activity (EEG) and somatosensory evoked potentials (SSEP) were also compared. Results indicate that the area infarcted in leukopenic rats was significantly less than infarcts generated in corresponding controls (21 +/- 16% vs. 70 +/- 16%). In addition, EEG was preserved in all leukopenic animals when compared to controls, both during ischemia and after reperfusion. The cortical peak component of the SSEP was also better preserved in the leukopenic animals both during ischemia and at 60 min of reperfusion. These results indicate white blood cell participation in the generation of cerebral damage in a model of global forebrain ischemia and reperfusion as indicated by TTC staining of cerebral infarcts.