Effect of purified rat and hamster hepatic glutathione S-transferases on the microsome mediated binding of aflatoxin B1 to DNA.
Effect of purified rat and hamster hepatic glutathione S-transferases on the microsome mediated binding of aflatoxin B1 to DNA.
复制标题
纯化的大鼠和仓鼠肝谷胱甘肽 S-转移酶对微粒体介导的黄曲霉毒素 B1 与 DNA 结合的影响。
DOI：
10.1016/0304-3835(86)90095-9
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发表时间：
1986
期刊：
影响因子：
9.7
通讯作者：
Lotlikar,PD
中科院分区：
文献类型：
--
作者：
Raj,HG;Prasanna,HR;Magee,PN;Lotlikar,PD
本文研究了用谷胱甘肽（GSH）亲和层析法纯化的大鼠和仓鼠肝细胞内谷胱甘肽S-转移酶对微粒体介导的黄曲霉毒素B_1（AFB_1）与DNA结合以及AFB_1 - 2，3-环氧化合物与GSH结合的影响。与以前用胞浆制备物进行的研究一样（Raj等（1984）Carcinogenesis 5，879），我们目前用纯化的GSH-转移酶进行的研究表明，与仓鼠结合的AFB 1-DNA的抑制活性比与大鼠结合的抑制活性高2-3倍。在抑制AFB_1-DNA结合的同时，AFB_1-GSH结合增加。谷胱甘肽转移酶的亚基组成表明优势的Yb和Ya亚基在仓鼠和大鼠，分别。本文讨论了谷胱甘肽转移酶在调节AFB_1-DNA结合和AFB_1诱发肝癌中的作用。
Rat and hamster liver cytosolic glutathione (GSH)S-transferases purified by GSH-affinity chromatography have been examined for their effects on the microsome mediated binding of aflatoxin B1(AFB1) to DNA and on the conjugation of AFB1-2,3-epoxide with GSH. Like previous studies with cytoslic preparations (Raj et al. (1984) Carcinogenesis 5, 879), our present study with purified GSHS-transferases showed 2–3-fold more inhibitory activity of AFB1-DNA binding with hamster than that with the rat. Concomitant with the inhibition of AFB1-DNA binding, increase in AFB1-GSH conjugation occurred. Subunit compositions of GSHS-transferases indicate preponderance of Yb and Ya subunits in the hamster and rat, respectively. The role of GSHS-transferases in modulating AFB1-DNA binding and AFB1induced hepatocarcinogenesis is discussed.