COMPLEXES OF YEAST RNA-POLYMERASE-II AND RNA ARE SUBSTRATES FAR TFIIS-INDUCED RNA CLEAVAGE
COMPLEXES OF YEAST RNA-POLYMERASE-II AND RNA ARE SUBSTRATES FAR TFIIS-INDUCED RNA CLEAVAGE
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DOI：
10.1016/0092-8674(94)90314-x
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发表时间：
1994-04-22
期刊：
影响因子：
64.5
通讯作者：
CHAMBERLIN, MJ
中科院分区：
文献类型：
--
作者：
JOHNSON, TL;CHAMBERLIN, MJ
在没有DNA的情况下，纯化的酵母RNA聚合酶II可以与RNA结合形成二元复合体。这种RNA-RNA聚合酶复合体中的RNA经历了以前被认为是与DNA的三元复合体中新生的RNA所特有的反应，包括依赖于TFIIS的切割和通过NTP的NMP 3‘末端加成的延伸。这两种反应都被α-氨甲酸甘油酯抑制。因此，根据几个标准，二元复合体中的RNA与聚合酶的结合方式与三元复合体中的非常相似，在三元复合体中，核苷酸加成的催化位置位于RNA的3‘-OH末端或其附近。这些发现与RNA聚合酶三元组完全的模型是一致的，在该模型中，RNA通过两个相距达10个核苷酸的蛋白质结合位点结合在3‘端。
In the absence of DNA, purified yeast RNA polymerase II can bind RNA to form a binary complex. RNA in such RNA-RNA polymerase complexes undergoes reactions previously thought to be unique to nascent RNA in ternary complexes with DNA, including TFIIS-dependent cleavage and elongation by 3'-terminal addition of NMP from NTP. Both of these reactions are inhibited by alpha-amanitin. Hence, by several criteria the RNA in binary complexes is bound to the polymerase in a manner quite similar to that in ternary complexes in which the catalytic site for nucleotide addition is positioned at or near the 3'-OH terminus of the RNA. These findings are consistent with a model for the RNA polymerase ternary complete in which the RNA is bound at the 3' terminus through two protein-binding sites located up to 10 nt apart.