Controlling Secretion in Artificial Cells with a Membrane AND Gate
Controlling Secretion in Artificial Cells with a Membrane AND Gate
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DOI：
10.1021/acssynbio.8b00435
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发表时间：
2019-06-01
影响因子：
4.7
通讯作者：
Kamat, Neha P.
中科院分区：
文献类型：
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作者：
Hilburger, Claire E.;Jacobs, Miranda L.;Kamat, Neha P.
将通道蛋白组装成囊泡膜是控制囊泡系统活性的有效策略。在这里，我们开发了一种膜和门，可以同时对脂肪酸和造孔通道蛋白做出反应，以诱导被包裹的货物的释放。我们探索了膜组成如何影响α-溶血素在磷脂囊泡中的功能性组装，作为油酸含量和α-溶血素浓度的函数。然后我们证明，当我们将油酸胶束添加到特定组成的磷脂泡囊中时，我们可以诱导α-溶血素组装。最后，我们证明了我们的膜和门可以连接到一个基因表达系统。我们的研究提供了一种新的方法，通过控制通道蛋白何时功能组装成合成囊泡来控制囊泡通透性的时间动态。此外，利用膜相关生物分子的膜与门引入了一种新的实现布尔逻辑的方法，应该补充遗传逻辑电路，并最终增强人工细胞系统的能力。
The assembly of channel proteins into vesicle membranes is a useful strategy to control activities of vesicle-based systems. Here, we developed a membrane AND gate that responds to both a fatty acid and a pore-forming channel protein to induce the release of encapsulated cargo. We explored how membrane composition affects the functional assembly of alpha-hemolysin into phospholipid vesicles as a function of oleic acid content and alpha-hemolysin concentration. We then showed that we could induce alpha-hemolysin assembly when we added oleic acid micelles to a specific composition of phospholipid vesicles. Finally, we demonstrated that our membrane AND gate could be coupled to a gene expression system. Our study provides a new method to control the temporal dynamics of vesicle permeability by controlling when the functional assembly of a channel protein into synthetic vesicles occurs. Furthermore, a membrane AND gate that utilizes membrane-associating biomolecules introduces a new way to implement Boolean logic that should complement genetic logic circuits and ultimately enhance the capabilities of artificial cellular systems.