IgM‐ and IgD‐bearing peripheral blood lymphocytes differentiate to IgM but not IgG or IgA immunoglobulin‐secreting cells
IgM‐ and IgD‐bearing peripheral blood lymphocytes differentiate to IgM but not IgG or IgA immunoglobulin‐secreting cells
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携带 IgM 和 IgD 的外周血淋巴细胞分化为 IgM，但不分化为 IgG 或 IgA 免疫球蛋白分泌细胞
DOI：
--
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发表时间：
1982
影响因子：
5.4
通讯作者：
P. Ralph
中科院分区：
文献类型：
--
作者：
O. Saiki;P. Ralph
耗尽人外周血单核细胞的表面IgM+或IgD+细胞，并测定有丝分裂原诱导分化为IgM、IgG和伊加类免疫球蛋白分泌细胞（ISC）。刺激剂包括T细胞依赖性poke weed有丝分裂原、B细胞有丝分裂原金黄色葡萄球菌菌株科万I，以及两种药物的组合，这两种药物可从所有正常供体中获得均匀、高水平的ISC。携带IgM或IgD的B淋巴细胞的耗尽会导致携带其他IG类别的细胞的损失，并阻断了对抗IgM和抗IgD的大部分促有丝分裂反应性。在这些耗尽的人群中，对科万I的排斥反应约为未分级单核细胞的20%。T细胞耗竭增加了对科万I和两种抗体制剂的促有丝分裂反应，表明它们是T非依赖性促有丝分裂原。IgD+细胞耗竭导致有丝分裂原诱导的IgM ISC部分丧失（未分离对照的22%-60%），但IgG或伊加ISC无丧失。携带IgM的细胞的消耗导致IgM ISC完全丧失，但IgG或伊加ISC没有丧失。我们先前证明，抗IgM抗体阻断了脾细胞中这三类免疫球蛋白IG分泌的有丝分裂原诱导，但仅阻断了血液单核细胞中IgM分泌。总之，这些结果表明，正常血液中对有丝分裂原有反应的大部分细胞成熟后产生IgG或伊加，属于IgM-、IgD-B细胞亚群，与脾脏中这些同种型分泌细胞的前体相反。因此，这些血液前体似乎比相应的脾细胞更成熟。
Human peripheral blood mononuclear cells were depleted of surface IgM+ or IgD+ cells and assayed for mitogen‐induced differentiation to immunoglobulin‐secreting cells (ISC) of IgM, IgG and IgA classes. Stimulatory agents included T cell‐dependent poke weed mitogen, B cell mitogen Staphylococcus aureus bacteria strain Cowan I, and a combination of the two which gives uniform, high levels of ISC from all normal donors. Depletion of either IgM‐ or IgD‐bearing B lymphocytes resulted in loss of cells bearing the other Ig class and blocked most of the mitogenic reactivity to anti‐IgM and anti‐IgD. Proliferative responses to Cowan I in these depleted populations were about 20% that of unfractionated mononuclear cells. Depletion of T cells increased the mitogenic response to Cowan I and to the two antibody preparations, showing that they are T‐independent mitogens. Depletion of IgD+ cells caused partial loss of mitogen‐induced IgM ISC (22%‐60% of unseparated controls) but no loss of IgG or IgA ISC. Depletion of IgM‐bearing cells caused complete loss of IgM ISC, but no loss of IgG or IgA ISC. We previously demonstrated that anti‐IgM antibody blocked mitogen induction of Ig secretion of these three classes in spleen cells, but only IgM secretion in blood mononuclear cells. Together, the results suggest that the majority of cells in normal blood responding to mitogens to mature to IgG or IgA production belong to IgM−, IgD− B cell subsets, in contrast to precursors of secreting cells for these isotypes in the spleen. Thus, these blood precursors appear to be more mature than the corresponding spleen cells.
DOI：
10.1016/0090-1229(81)90194-x
发表时间：
1981
期刊：
Clinical immunology and immunopathology
影响因子：
--
作者：
Levitt,D;Dagg,MK
通讯作者：
Dagg,MK
人外周血中抗破伤风类毒素抗体产生细胞的多个亚群在大小、膜受体的表达和有丝分裂原反应性方面有所不同。
DOI：
--
发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Thiele,CJ;Morrow,CD;Stevens,RH
通讯作者：
Stevens,RH