CORRELATION OF THEORETICAL AND EXPERIMENTAL YIELDS OF PHENYLALANINE FROM NON-GROWING CELLS OF A REC ESCHERICHIA-COLI STRAIN
CORRELATION OF THEORETICAL AND EXPERIMENTAL YIELDS OF PHENYLALANINE FROM NON-GROWING CELLS OF A REC ESCHERICHIA-COLI STRAIN
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DOI：
10.1016/0168-1656(88)90043-0
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发表时间：
1988-04-01
影响因子：
4.1
通讯作者：
HAGGSTROM, L
中科院分区：
文献类型：
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作者：
FORBERG, C;ELIAESON, T;HAGGSTROM, L
含有营养缺陷型质粒的大肠杆菌突变体（E. coli）产生苯丙氨酸。在分批培养中研究了携带基因aroF、pheAFBR、TcR和ApR的大肠杆菌W3110 Δ trp、Δ tyr、Δ phe/pJN 6。由于酪氨酸耗尽后反馈抑制的释放，在稳定期产生苯丙氨酸。接种物的生理状态严重影响苯丙氨酸的产生动力学。苯丙氨酸的比产率在停止生长后立即达到最大值，并且生产至少持续40 h。在此期间的间隔期间，苯丙氨酸生产的比速率保持恒定，而葡萄糖消耗的比速率降低，导致苯丙氨酸从葡萄糖的产率（0.27 g g-1），其对应于估计的最大理论产率系数。对碳平衡的详细分析表明，在这一时期之前和之后，形成了一种与二氧化碳生产无关的副产品。在特定的时间间隔内，所有消耗的葡萄糖最终以产生的苯丙氨酸和二氧化碳的形式回收。这种情况只能通过非生长细胞来实现。
Phenylalanine production from an auxotrophic plasmid-harboring Escherichia coli mutant (E. coli W3110 .DELTA.trp, .DELTA.tyr, .DELTA.phe/pJN6 carrying the genes aroF, pheAFBR, TcR and ApR) was studied in batch cultures. Phenylalanine was produced in the stationary phase due to the release of feed-back inhibition after depletion of tyrosine. The physiological state of the inoculum seriously affected the kinetics of phenylalanine production. The specific rate of phenylalanine production reached a maximum directly after cessation of growth and the production continued for at least 40 h. During an interval in this period the specific rate of phenylalanine production remained constant while the specific rate of glucose consumption decreased resulting in a yield of phenylalanine from glucose (0.27 g g-1) that corresponded to the estimated maximum theoretical yield coefficient. Detailed analyses of carbon balances showed that before and after this period a by-product not coupled to carbon dioxide production was formed. During the specific interval all glucose consumed was finally recovered in produced phenylalanine and carbon dioxide. This situation can only be achieved with non-growing cells.