Whole-genome sequencing of 234 bulls facilitates mapping of monogenic and complex traits in cattle
Whole-genome sequencing of 234 bulls facilitates mapping of monogenic and complex traits in cattle
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DOI：
10.1038/ng.3034
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发表时间：
2014-08-01
期刊：
影响因子：
30.8
通讯作者：
Hayes, Ben J.
中科院分区：
文献类型：
--
作者：
Daetwyler, Hans D.;Capitan, Aurelien;Hayes, Ben J.
1000头公牛基因组项目通过提供关键祖先公牛的注释序列变体和基因型，支持加速家养牛遗传增益率的目标，同时考虑动物健康和福利。在1000头公牛基因组项目的第一阶段，我们对234头牛的全基因组进行了测序，平均覆盖率为8.3倍。该测序包括来自全球Holstein-Friesian种群的129个个体，来自Fleckvieh品种的43个个体和来自泽西品种的15个个体的数据。我们总共鉴定了2830万个变异体，每个个体的每个酶平均有1.44个杂合位点。我们证明了使用这个数据库在确定隐性突变潜在的胚胎死亡和显性突变潜在的致命chordrodysplasia。我们还进行了产奶量和卷毛的全基因组关联研究，使用插补序列变异，并确定了与牛的这些性状相关的变异。
The 1000 bull genomes project supports the goal of accelerating the rates of genetic gain in domestic cattle while at the same time considering animal health and welfare by providing the annotated sequence variants and genotypes of key ancestor bulls. In the first phase of the 1000 bull genomes project, we sequenced the whole genomes of 234 cattle to an average of 8.3-fold coverage. This sequencing includes data for 129 individuals from the global Holstein-Friesian population, 43 individuals from the Fleckvieh breed and 15 individuals from the Jersey breed. We identified a total of 28.3 million variants, with an average of 1.44 heterozygous sites per kilobase for each individual. We demonstrate the use of this database in identifying a recessive mutation underlying embryonic death and a dominant mutation underlying lethal chrondrodysplasia. We also performed genome-wide association studies for milk production and curly coat, using imputed sequence variants, and identified variants associated with these traits in cattle.