Identification of a phosphorus-solubilizing Tsukamurella tyrosinosolvens strain and its effect on the bacterial diversity of the rhizosphere soil of peanuts growth-promoting
Identification of a phosphorus-solubilizing Tsukamurella tyrosinosolvens strain and its effect on the bacterial diversity of the rhizosphere soil of peanuts growth-promoting
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DOI：
10.1007/s11274-021-03078-3
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发表时间：
2021-07-01
影响因子：
4.1
通讯作者：
Long, Changmei
中科院分区：
文献类型：
--
作者：
Zhang, Hong;Han, Lizhen;Long, Changmei
解磷微生物广泛存在于植物根际土壤中，但稀有放线菌的解磷和多重促生特性的研究报道较少。从茶树根际土壤中分离到一株解磷的Tsukamurella tyrosinosolvens P9菌株。该菌株在不同碳源和氮源下的溶磷能力不同，以葡萄糖和硝酸钾为营养源时，可溶性磷含量为442.41 mg/L。分泌各种有机酸，如乳酸、马来酸、草酸等，培养液pH值与可溶性磷含量呈极显著负相关。此外，该菌株还具有多种促生长特性，IAA含量为37.26 μ g/mL，铁载体相对含量为72.01%。盆栽试验表明，P9菌株对花生幼苗的生长有明显的促进作用。用P9菌株接种花生根际土壤，对土壤细菌群落结构进行了研究。结果表明，接种组与对照组的α多样性指数差异不显著，但P9处理组改变了细菌群落的组成，增加了有益微生物和功能微生物的相对丰度，其中噬几丁质菌科的相对丰度显著增加，黄腐杆菌属（Flavihumibacter）、枝杆菌属（Ramlibacter）和微腐菌属（Microvirga）的相对丰度显著增加。特别地，Tsukamurella tyrosinosolvens仅在接种组的根际中检测到。本研究不仅发现了T.本文介绍了溶磷菌P9菌株及其可能的溶磷机理，并有望为生物肥料提供优良的菌株资源。[图形]。
Phosphate solubilizing microorganisms widely exist in plant rhizosphere soil, but report about the P solubilization and multiple growth-promoting properties of rare actinomycetes are scarce. In this paper, a phosphate solubilizing Tsukamurella tyrosinosolvens P9 strain was isolated from the rhizosphere soil of tea plants. Phosphorus-dissolving abilities of this strain were different under different carbon and nitrogen sources, the soluble phosphorus content was 442.41 mg/L with glucose and potassium nitrate as nutrient sources. The secretion of various organic acids, such as lactic acid, maleic acid, oxalic acid, etc., was the main mechanism for P solubilization and pH value in culture was very significant negative correlation with soluble P content. In addition, this strain had multiple growth-promoting characteristics with 37.26 mu g/mL of IAA and 72.01% of siderophore relative content. Under pot experiments, P9 strain improved obviously the growth of peanut seedlings. The bacterial communities of peanut rhizoshpere soil were assessed after inoculated with P9 strain. It showed that there was no significant difference in alpha-diversity indices between the inoculation and control groups, but the P9 treatment group changed the composition of bacterial communities, which increased the relative abundance of beneficial and functional microbes, which relative abundances of Chitinophagaceae at the family level, and of Flavihumibacter, Ramlibacter and Microvirga at the genus level, were all siginificant increased. Specially, Tsukamurella tyrosinosolvens were only detected in the rhizosphere of the inoculated group. This study not only founded growth-promoting properties of T. tyrosinosolvens P9 strain and its possible phosphate solublizing mechanism, but also expected to afford an excellent strain resource in biological fertilizers.[GRAPHICS].